Beta-Lactamase-BS (broad-spectrum) Inactivates Beta-Lactams

March 7, 2013 Categories AG Scientific Blog

Beta-Lactamase-BS (broad-spectrum) Inactivates Beta-Lactams

Description

β-lactamase-broad spectrum has broad scope of activity against both penicillins (penicillinase or Beta I activity), up to 5th generation cephalosporins (cephalosporinase or Beta II activity), as well as, the Carbapenems:  Ertapenem, Imipenem, Meropenem.l-1163_laboratory_detection_of_extended-spectrum_β-lactamases

β-LACTAMASE-BS is a freeze-dried product containing buffer salts and zinc. β-LACTAMASE-BS has a broader spectrum activity of complete inactivation of a broad range of cephalosporins and penicillins.

β-LACTAMASE-BS has been demonstrated to inactivate the following PENICILLINS:

Amdinocillin, Amoxicillin, Azlocillin, Benzylpenicillin, Carbenicillin, Cloxacillin, Flucloxacillin, Methicillin, Mezlocillin, Nafcillin, Oxacillin, Penicillin, Piperacillin, Ticarcillin.

β-LACTAMASE-BS has been demonstrated to inactivate the following CEPHALOSPORINS:

1ST GENERATION: Cefadroxil, Cefalexin,Cefaloridine, Cefazolin.

2ND GENERATION: Cefaclor, Cefamandole, Cefmetazole, Cefonicid, Cefotetan, Cefoxitin, Cefprozil, Cefuroxime.

3RD GENERATION: Cefdinir, Cefixime, Cefopdoxime, Cefotaxime, Cefsulodin, Ceftazidime, Ceftibuten, Ceftiofur, Ceftizoxime, Ceftriaxone, Moxalactum.

4TH GENERATION: Cefepime, Cefpirome_ag_scientific_beta-lactamase-bs_,_cephalosporin_general_structure,_beta_lactamase-broad_spectrum_inactivates_upto_5th_generation_cephalosporins_including_ceftobiprole

5TH GENERATION: Ceftobiprole

β-LACTAMASE-BS has been demonstrated to inactivate the following

CARBAPENEMS: Ertapenem, Imipenem, Meropenum.

ag_scientific_beta-lactamase-bs_,_betalactam_ring_structure,_beta_lactamase-broad_spectrum_inactivates_ertapenem,imipenem_and_meropenem

Beta-Lactamase-BS

β-Lactamase-Broad Spectrum Applications:

1. Testing Blood Culture Sterility

Blood cultures are routinely prepared in order to test for bacterial infection. False negative results might be obtained where the blood sample contains antibiotics. Incorporation of β-lactamase in the culture medium will overcome this problem when cephalosporins /penicillins are present.

2. Testing for Contamination of Drugs by Antibiotics

US Code of Federal regulations states that “If a reasonable possibility exists that a non-penicillin drug product has been exposed to cross-contamination with penicillin, the non-penicillin drug product shall be tested for the presence of penicillin (21 CFR 211.176, Penicillin Contamination, FDA, BY-lines No. 8 November 1977).

3. Environmental Monitoring of Antibiotic Manufacturing Areas

Contact plates, settle plates and air monitoring systems for testing of aseptic conditions in antibiotic manufacturing facilities need to be manufactured with agar medium for neutralization of antibiotic. This is achieved by the addition of Penicillinase or β-Lactamase to the medium. Through this, any antibiotic residues are hydrolyzed and microbial contamination can be detected.

4. Sterility Testing of Bulk Antibiotics

US Pharmacopeia (USP) Chapter 71 and EP Section 2.6 describe sterility testing of bulk antibiotics, which should be shown to be free from microbial contamination. The testing requires the removal of significant amounts of active antibiotic from solution by combined filtration and the use of penicillinase or β-Lactamase, The resulting solution is tested for the (lack of) growth of microbes. USP specifies that the amount of Penicillinase or β-Lactamase used in this removal process should be verified using a microbial challenge solution in a control sample.

β-Lactam Overuse:

In light of the overuse of β-lactam antibiotics and resulting resistance, new and novel broader spectrum β-lactamase (L-2467) is an essential tool. For environmental and sterility monitoring of antibiotic manufacturing facilities under FDA (21 CFR211.176 and CFR436.20), broader spectrum β-Lactamase is now a requirement.

Enhanced Features:

FULL INACTIVATION: of a broad range of β-Lactams including cephalosporin: Cefepime, Cefoxitin, Cefradine and Ceftaxidime. Other commercially available enzymes do not degrade as efficiently or as comprehensively.
HIGHER STABILITY: Retains <90% of its activity after 4 hours at 50°C, which means no need to compensate for loss of enzyme activity during media preparation for applications utilizing sterile plates.

You can get more information by signing up for our Beta-Lactamase E-Guide. Get your copy below:

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