ABTS

SKU
A-2179
  • CAS:30931-67-0
  • Formula:C18H18N4O6S4
  • MW:514.6
  • Appearance:Liquid

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Product Name Qty
ABTS
100 mL
$179.00

Description

ABTS [2,2'-Azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid)] is a safe and sensitive substrate for horseradish peroxidase (HRP)-based ELISA (enzyme-linked immunosorbent assay). It is useful in ELISA assay because of its high sensitivity, specificity, and reproducibility.

The substrate generates a soluble, green product. It can be read spectrophotometrically at 405 nm.

ABTS in decolorization assays appears blue in the presence of sodium or potassium persulfate or metmyoglobin, however, it decolorizes upon incubation with antioxidants including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants. By this mechanism, it determines the antioxidant activity.

 

Applications

• Substrate for ELISA

• Used to assess antioxidant activity in TEAC assays

 

References

“Evaluation of ELISA With ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline Sulfonic Acid), as the Substrate of Peroxidase and Its Application to the Diagnosis of Schistosomiasis”

“Antioxidant activity applying an improved ABTS radical cation decolorization assay”

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

More Information
Alternate Name/Synonyms
2,2'-Azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid); EINECS 250-396-6; ABTS Diammonium; 2,2'-Azino-di(3-ethyl-benzthiazoline sulphonic acid (6)), ammonium salt; Ammonium 2,2'-(hydrazine-1,2-diylidene)bis(3-ethyl-2,3-dihydrobenzo[d]thiazole-6-sulfonate)
SKU
A-2179
CAS #
30931-67-0
Chemical Name
diazanium;(2Z)-3-ethyl-2-[(Z)-(3-ethyl-6-sulfonato-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonate
Chemical Formula
C18H18N4O6S4
Molecular Weight
514.6
Appearance
Liquid
Storage Temp
2-8 ℃
USE
A peroxidase substrate suitable for use in ELISA procedures
MDL NUMBER
MFCD00010404
CHEMACX
X1079584-9
INCHI
InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
SMILES
CCN1/C(=N/N=C/2SC3=C(N2CC)C=CC(=C3)S(=O)(=O)[O-])/SC4=C1C=CC(=C4)S(=O)(=O)[O-].[NH4+].[NH4+]
RTECS
DL7002000
Handling
Keep away from heat. Keep away from sources of ignition. Empty containers pose a fire risk; evaporate the residue under a fume hood. Ground all equipment containing material. Do no breathe gas/fumes/vapors/spray. Wear suitable protective clothing. If you feel unwell, seek medical attention and show the label when possible. Avoid contact with skin and eyes.
Certificate of Analysis 1
GHS PICTOGRAMS
Handling Keep away from heat. Keep away from sources of ignition. Empty containers pose a fire risk; evaporate the residue under a fume hood. Ground all equipment containing material. Do no breathe gas/fumes/vapors/spray. Wear suitable protective clothing. If you feel unwell, seek medical attention and show the label when possible. Avoid contact with skin and eyes.
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