Buffers

AG Scientific supplies reliable, high-quality Biological Buffers

AG Scientific offers biological buffers to assist in a variety of life science research applications. Most biological processes are dependent on a stable pH and even minor changes can cause inefficiencies, complications, and impact yield and cost. Our buffers are specifically designed to maintain pH and resist change within a very limited scope. Each buffer neutralizes intracellular and extracellular pH within a very specific range, despite the presence of both internal and external influences.

To obtain leading results in research that requires optimal pH, AG Scientific supplies reliable, high-quality buffers for any application. Our buffering reagents are designed for use in enzyme assays, gel electrophoresis, and protein isolation.

 

A buffer is a solution that neutralizes pH despite the presence of small amounts of an acid or base that may attempt to change the pH of a substance. The use of buffers is particularly significant in processes and reactions that require a stable pH. Buffers consist of a weak conjugate acid-base pair--meaning a weak acid and its conjugate base or a weak base and its conjugate acid. The combined components create the presence of an equilibrium which in turn balances the pH of the substance. The composition of the buffer is dependent on the desired pH of the process being tested or studied.

Buffers used specifically in biological research were studied and popularized by Norman Good, et al., and are commonly referred to today as Good buffers. These organic buffering compounds and solutions provide maximum buffering capacity between pH 6 and 8. Their use was a breakthrough in 1966 when biological research was previously stunted by reactive and even toxic buffers. Some of the more commonly used Good Buffers, in order of increasing pKa, include MES, ADA, PIPES, ACES, cholamine chloride, BES, TES, HEPES, acetamidoglycine, tricine, glycinamide, and Bicine. These buffers were selected on the basis of particular criteria including pH, solubility, membrane impermeability, low ionic/salt effects, low cationic complex activity, stability, optical absorbance, and ease of preparation. They also help maintain a nearly ideal pH state and are perfect for applications that require rigid regulation of buffer composition.

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Items 1-50 of 84

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  1. 3X SDS-PAGE Loading Buffer
    3X SDS-PAGE Loading Buffer
    S-2429

    Starting at $99.04

  2. Ammonium Acetate
    Ammonium Acetate
    A-2743

    Starting at $41.73

  3. bis-TRIS
    bis-TRIS
    B-2433

    Starting at $89.16

  4. BIS-TRIS Propane chemical structure | CAS 64431-96-5 | Biological Buffer | AG Scientific, Inc
    BIS-TRIS Propane
    B-2432

    Starting at $135.22

  5. Boric Acid, ACS Grade | CAS 10043-35-3 | AG Scientific, Inc.
    Boric Acid, ACS Grade
    B-2601

    Starting at $58.60

  6. CAPS
    CAPS
    C-1033

    Starting at $37.28

  7. CHAPS 10% Solution, Sterile Filtered | Chemical Structure | Biodetergent | AG Scientific, Inc.
    CHAPS 10% Solution, Sterile-Filtered
    C-1020

    Starting at $52.16

  8. CHAPS | Biodetergent | CAS 75621-03-3 | AG Scientific Inc.
    CHAPS Zwitterionic, High Purity
    C-1019

    Starting at $54.26

  9. CHES Buffer
    CHES Buffer
    C-2431

    Starting at $62.60

  10. Citric Acid chemical structure | CAS 5949-29-1 | AG Scientific, Inc.
    Citric Acid Monohydrate, ACS Grade
    C-2763

    Starting at $65.98

  11. D-(-)-Salicin
    D-(-)-Salicin
    D-2430

    Starting at $93.16

  12. DTT, Dithiothreitol | CAS 3483-12-3 | Reducing Agent | AG Scientific
    Dithiothreitol (DTT)
    C-1029

    Starting at $34.42

  13. EDTA 0.5M Solution (pH 8.0)
    EDTA 0.5M Solution (pH 8.0)
    E-2481

    Starting at $78.26

  14. EDTA Disodium Salt
    EDTA Disodium Salt
    E-2483

    Starting at $73.19

  15. EDTA Ferric Sodium Salt
    EDTA Ferric Sodium Salt
    E-2485

    Starting at $83.33

  16. EDTA, Tetrasodium Salt, Anhydrous
    EDTA, Tetrasodium Salt, Anhydrous
    E-2487

    Starting at $73.19

  17. EGTA (Ethylene Glycol Tetraacetic Acid)
    EGTA (Ethylene Glycol Tetraacetic Acid)
    E-2489

    Starting at $78.26

  18. EGTA 0.5M Solution pH 8.0
    EGTA 0.5M Solution pH 8.0
    E-2491

    Starting at $75.13

  19. Formalin 10% Neutral Buffered Solution
    Formalin 10% Neutral Buffered Solution
    F-2467

    Starting at $49.52

  20. Glycerol, ACS Grade
    Glycerol, ACS Grade
    G-2583

    Starting at $36.52

  21. Glycine
    Glycine
    G-2585

    Starting at $209.01

  22. Guanidine HCl, 8M solution
    Guanidine HCl, 8M solution
    G-2587

    Starting at $903.46

  23. Guanidine Hydrochloride
    Guanidine Hydrochloride
    G-2589

    Starting at $172.16

  24. HEPES Buffered Saline Solution 2X
    HEPES Buffered Saline Solution 2X
    H-2430

    Starting at $72.77

  25. HEPES, Free Acid chemical structure | CAS 7365-45-9 | AG Scientific, Inc.
    HEPES, Free Acid
    H-2431

    Starting at $83.47

  26. HEPES, sodium salt
    HEPES, sodium salt
    H-2432

    Starting at $0.00

  27. HEPPS | CAS 16052-06-5 | Biological Buffer with Useful pH Range | AG Scientific, Inc.
    HEPPS
    H-1030

    Starting at $118.05

  28. Imidazole, MegaPure
    Imidazole, MegaPure
    I-2360

    Starting at $145.82

  29. Killer TRAIL Storage & Dilution Buffer
    Killer TRAIL Storage & Dilution Buffer
    T-1065

    Starting at $79.24

  30. Laemmli Buffer Reducing, 4X Solution
    Laemmli Buffer Reducing, 4X Solution
    L-2411

    Starting at $78.84

  31. Lysis Buffer for Plasmid DNA
    Lysis Buffer for Plasmid DNA
    I-2619

    Starting at $134.05

  32. MES Monohydrate
    MES Monohydrate
    M-1219

    Starting at $67.81

  33. MES, Anhydrous, Ultrapure chemical structure | CAS 4432-31-9 | Zwitterionic Buffer | AG Scientific, Inc.
    MES, Anhydrous
    M-2699

    Starting at $52.16

  34. MOPS, Free Acid
    MOPS, Free Acid
    M-2721

    Starting at $73.03

  35. MOPS, Sodium Salt
    MOPS, Sodium Salt
    M-2413

    Starting at $78.26

  36. MOPSO
    MOPSO
    M-2414

    Starting at $70.94

  37. Neutralization Buffer for Plasmid DNA
    Neutralization Buffer for Plasmid DNA
    N-2727

    Starting at $300.95

  38. NP-40 Lysis High Salt Buffer Solution
    NP-40 Lysis High Salt Buffer Solution
    N-2410

    Starting at $56.80

  39. PBS 10x Powder with Calcium and Magnesium
    PBS 10x Powder with Calcium and Magnesium
    P-2825

    Starting at $238.03

  40. PBS-Tween Tablets (100 mL)
    PBS-Tween Tablets (100 mL)
    P-2387

    Starting at $135.04

  41. Phosphate Buffered Saline (PBS) 10X Solution
    Phosphate Buffered Saline (PBS) 10X Solution
    P-2425

    Starting at $47.65

  42. Phosphate Buffered Saline (PBS) 1X Sterile Solution
    Phosphate Buffered Saline (PBS) 1X Sterile Solution
    P-2426

    Starting at $45.73

  43. PIPES, Free Acid chemical structure | CAS 5625-37-6 | Buffering Agent | AG Scientific, Inc.
    PIPES, Free Acid
    P-2429

    Starting at $78.93

  44. Potassium Bicarbonate, ACS Grade
    Potassium Bicarbonate, ACS Grade
    P-2873

    Starting at $37.26

  45. Potassium Chloride
    Potassium Chloride
    P-2877

    Starting at $52.16

  46. Potassium Hydroxide
    Potassium Hydroxide
    P-2881

    Starting at $53.64

  47. PVDF Membrane Stripping Buffer-(4X)
    PVDF Membrane Stripping Buffer-(4X)
    P-2903

    Starting at $70.01

  48. Resuspension Buffer for Plasmid DNA
    Resuspension Buffer for Plasmid DNA
    R-2649

    Starting at $211.57

  49. RIPA Buffer 2X Solution
    RIPA Buffer 2X Solution
    R-2417

    Starting at $80.35

  50. Separating or Resolving Buffer 4X Solution
    Separating or Resolving Buffer 4X Solution
    S-2420

    Starting at $46.18

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