Buffers

AG Scientific supplies reliable, high-quality Biological Buffers

AG Scientific offers biological buffers to assist in a variety of life science research applications. Most biological processes are dependent on a stable pH and even minor changes can cause inefficiencies, complications, and impact yield and cost. Our buffers are specifically designed to maintain pH and resist change within a very limited scope. Each buffer neutralizes intracellular and extracellular pH within a very specific range, despite the presence of both internal and external influences.

To obtain leading results in research that requires optimal pH, AG Scientific supplies reliable, high-quality buffers for any application. Our buffering reagents are designed for use in enzyme assays, gel electrophoresis, and protein isolation.

 

A buffer is a solution that neutralizes pH despite the presence of small amounts of an acid or base that may attempt to change the pH of a substance. The use of buffers is particularly significant in processes and reactions that require a stable pH. Buffers consist of a weak conjugate acid-base pair--meaning a weak acid and its conjugate base or a weak base and its conjugate acid. The combined components create the presence of an equilibrium which in turn balances the pH of the substance. The composition of the buffer is dependent on the desired pH of the process being tested or studied.

Buffers used specifically in biological research were studied and popularized by Norman Good, et al., and are commonly referred to today as Good buffers. These organic buffering compounds and solutions provide maximum buffering capacity between pH 6 and 8. Their use was a breakthrough in 1966 when biological research was previously stunted by reactive and even toxic buffers. Some of the more commonly used Good Buffers, in order of increasing pKa, include MES, ADA, PIPES, ACES, cholamine chloride, BES, TES, HEPES, acetamidoglycine, tricine, glycinamide, and Bicine. These buffers were selected on the basis of particular criteria including pH, solubility, membrane impermeability, low ionic/salt effects, low cationic complex activity, stability, optical absorbance, and ease of preparation. They also help maintain a nearly ideal pH state and are perfect for applications that require rigid regulation of buffer composition.

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  1. Sodium Chloride
    Sodium Chloride
    S-2843

    Starting at $67.75

  2. Sodium Chloride 5M Solution
    Sodium Chloride 5M Solution
    S-2440

    Starting at $53.64

  3. Sodium Chloride, ACS Grade
    Sodium Chloride, ACS Grade
    S-2845

    Starting at $77.46

  4. Sodium Formate chemical structure | CAS 141-53-7 | AG Scientific, Inc.
    Sodium Formate, ACS Grade
    S-2077

    Starting at $37.55

  5. Sodium Phosphate Dibasic Anhydrous
    Sodium Phosphate Dibasic Anhydrous
    S-2859

    Starting at $43.24

  6. Sodium Phosphate Dibasic Heptahydrate
    Sodium Phosphate Dibasic Heptahydrate
    S-2857

    Starting at $46.18

  7. Sodium Phosphate Monobasic Anhydrous
    Sodium Phosphate Monobasic Anhydrous
    S-2867

    Starting at $46.18

  8. Sodium Phosphate Monobasic Monohydrate
    Sodium Phosphate Monobasic Monohydrate
    S-2863

    Starting at $43.83

  9. Sodium Phosphate Monobasic, Monohydrate, ACS Grade
    Sodium Phosphate Monobasic, Monohydrate, ACS Grade
    S-2865

    Starting at $43.24

  10. SSC buffer, 20X powder
    SSC buffer, 20X powder
    S-2460

    Starting at $99.70

  11. SSC buffer, 20X solution
    SSC buffer, 20X solution
    S-2461

    Starting at $119.01

  12. SSPE buffer 20X Solution pH 7.4
    SSPE buffer 20X Solution pH 7.4
    S-2462

    Starting at $38.73

  13. SSPE buffer, 20X
    SSPE buffer, 20X
    S-2463

    Starting at $69.92

  14. Stacking Buffer 4X Solution
    Stacking Buffer 4X Solution
    S-2464

    Starting at $46.18

  15. STE Buffer 1X Solution
    STE Buffer 1X Solution
    S-2465

    Starting at $38.73

  16. STET Buffer 1X Solution
    STET Buffer 1X Solution
    S-2466

    Starting at $38.73

  17. TAE (TRIS-Acetate-EDTA), 10X Solution
    TAE (TRIS-Acetate-EDTA), 10X Solution
    T-2751

    Starting at $38.73

  18. TAPS, Free Acid
    TAPS, Free Acid
    T-2405

    Starting at $35.81

  19. TBE (TRIS-Borate-EDTA) Buffer 10X Powder
    TBE (TRIS-Borate-EDTA) Buffer 10X Powder
    T-2406

    Starting at $119.99

  20. TBS (TRIS Buffered Saline), 10X Solution
    TBS (TRIS Buffered Saline), 10X Solution
    T-2747

    Starting at $43.24

  21. TBS / Tween20 10X Solution
    TBS / Tween20 10X Solution
    T-2407

    Starting at $52.16

  22. TBST Buffer, 10X solution, pH 7.4
    TBST Buffer, 10X solution, pH 7.4
    T-2786

    Starting at $115.00

  23. TE Buffer 20X Solution pH 8.0
    TE Buffer 20X Solution pH 8.0
    T-2409

    Starting at $38.73

  24. TGS (TRIS-Glycine-SDS) Buffer 10X Powder
    TGS (TRIS-Glycine-SDS) Buffer 10X Powder
    T-2709

    Starting at $46.18

  25. TGS (TRIS-Glycine-SDS) Buffer 10X Solution
    TGS (TRIS-Glycine-SDS) Buffer 10X Solution
    T-2711

    Starting at $59.50

  26. Tricine
    Tricine
    T-2733

    Starting at $59.50

  27. Tricine-SDS Sample Buffer 2X Solution
    Tricine-SDS Sample Buffer 2X Solution
    T-2735

    Starting at $38.73

  28. TRIS Acetate, Ultra Pure
    TRIS Acetate, Ultra Pure
    T-2745

    Starting at $53.64

  29. Tris Base
    Tris Base
    T-1178

    Starting at $89.00

  30. TRIS Hydrochloride
    TRIS Hydrochloride
    T-1176

    Starting at $84.79

  31. TRIS Hydrochloride Solution
    TRIS Hydrochloride Solution
    T-2468

    Starting at $87.65

  32. TRIS-CAPS Buffer 10X Solution
    TRIS-CAPS Buffer 10X Solution
    T-2753

    Starting at $46.18

  33. TRIS-Tricine-SDS Running Buffer 10X Solution
    TRIS-Tricine-SDS Running Buffer 10X Solution
    T-2755

    Starting at $53.64

  34. Triton Lysis Buffer Solution
    Triton Lysis Buffer Solution
    T-2757

    Starting at $53.64

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