AMP (Agarose) 4B-CL (Lyophilized)

SKU
A-2917
  • Appearance:Lyophilized
Product Name Qty
AMP(Agarose) 4B-CL (Lyophilized)
1 g
$379.53

Applications:

AMP interacts strongly with NAD+-dependent dehydrogenases and ATP-dependent enzymes. Selective elution with gradients of NAD+ or NADP+ allows the resolution of complex mixtures of dehydrogenase isoenzymes using 5`-AMP Separopore®.

The production method involves alkylation of the nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N6-carboxymethyl derivative with subsequent condensation using 1,6-diaminohexane to give N6-[(6-aminohexyl)carbamoylmethyl)].

 

Technical Specifications:

  • Ligand: Adenosine Monophosphate (AMP)
  • Matrix: Separopore® 4B-CL (highly crosslinked agarose beads, 4%).
  • Particle size range: 52 – 165 µm
  • Matrix activation: Epoxy
  • Matrix attachment: N6
  • Spacer arm: 1, 6-diaminohexane
  • Ligand density: 2 µmol AMP / ml drained gel
  • Binding capacity: ~ 5-7 mg lactate dehydrogenase / ml drained gel
  • pH stability: 4 – 10
  • Flow rate specifications: 70 – 140 cm / h.
  • Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents
  • Physical stability: Negligible volume variation due to changes in pH or ionic strength
  • Supplied as lyophilized powder and 1g yields 8-10 ml of gel
More Information
Alternate Name/Synonyms
AMP 4B-CL NAD+-dependent dehydrogenases, AMP 4B-CL ATP-dependent enzymes, Adenosine Monophosphate, AMP from Agarose, buy AMP 4B-CL, AMP 4B-CL vendor, AMP 4B-CL supplier, AMP 4B-CL price
SKU
A-2917
Appearance
Lyophilized
Storage Temp
-20°C
Hazard Image
Yes
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