AMP (Agarose) 4B-CL (Lyophilized)
AMP interacts strongly with NAD+-dependent dehydrogenases and ATP-dependent enzymes. Selective elution with gradients of NAD+ or NADP+ allows the resolution of complex mixtures of dehydrogenase isoenzymes using 5`-AMP Separopore®.
The production method involves alkylation of the nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N6-carboxymethyl derivative with subsequent condensation using 1,6-diaminohexane to give N6-[(6-aminohexyl)carbamoylmethyl)].
- Ligand: Adenosine Monophosphate (AMP)
- Matrix: Separopore® 4B-CL (highly crosslinked agarose beads, 4%).
- Particle size range: 52 – 165 µm
- Matrix activation: Epoxy
- Matrix attachment: N6
- Spacer arm: 1, 6-diaminohexane
- Ligand density: 2 µmol AMP / ml drained gel
- Binding capacity: ~ 5-7 mg lactate dehydrogenase / ml drained gel
- pH stability: 4 – 10
- Flow rate specifications: 70 – 140 cm / h.
- Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents
- Physical stability: Negligible volume variation due to changes in pH or ionic strength
- Supplied as lyophilized powder and 1g yields 8-10 ml of gel