Aphidicolin

Product Name	Qty
Aphidicolin 1 mg $102.00
Aphidicolin 5 mg $402.90
Aphidicolin 25 mg $1,524.90

Description

Aphidicolin is a reversible inhibitor of nuclear DNA replication in eukaryotes and some animal viruses. It interferes with dCTP-specific binding sites on DNA polymerase-α. It also works on other DNA polymerases, however it does not work on DNA polymerase-β.

Viruses inducing DNA polymerase-α, such as Herpes simplex, can be affected by aphidicolin because of its mechanism. It could be used for clinical study.

Isolated from Nigrospora oryzae.

Applications

• Control of cell proliferation

• DNA pol-β studies

References

• “Aphidicolin: a specific inhibitor of nuclear DNA replication in eukaryotes”

• “Effect of aphidicolin on viral and human DNA polymerases”

• "DNA Replication Dynamics of the GGGGCC Repeat of the C9orf72 Gene"

• "Structural basis for inhibition of DNA replication by aphidicolin"

Specifications

More Information

Alternate Name/Synonyms

APC; ICI 69653; NSC 234714

SKU

A-1026

CAS #

38966-21-1

Chemical Name

(1S,2S,5R,6R,7R,10S,12R,13R)-6,13-bis(hydroxymethyl)-2,6-dimethyltetracyclo[10.3.1.01,10.02,7]hexadecane-5,13-diol

Chemical Formula

C₂₀H₃₄O₄

Molecular Weight

338.5 Da

Appearance

White Powder

Purity

>99% (TLC)

Solubility

Methanol: Clear colorless solution at 10 mg/ml

Melting Point

230°C

Preparation

N/A

Storage Temp

+4°C

THERAPEUTIC AREA

Not Available

USE

For research use only. Not intended for diagnostic or therapeutic use. Aphidicolin is a tetracyclic diterpene with antibiotic, antiviral and antimitotic properties by blocking the cell cycle at early S-phase. This property has been used to synchronise cell division and is useful as a tool in cell differentiation research. Aphidicolin induces apoptosis, prolongs the half-life of DNA methyltransferase, is active against Leishmania parasites and acts synergistically with the antitumor agents, vincristine and doxorubicin.

Merck Index

Merck Index: 13.734.2001

MDL NUMBER

MFCD00083214

CHEMACX

X1001937-7

INCHI

InChI=1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1

SMILES

C[C@]12CC[C@H]([C@@]([C@@H]1CC[C@@H]3[C@@]24CC[C@@]([C@H](C3)C4)(CO)O)(C)CO)O

Peptide sequence

Not Applicable

RTECS

PB9185000

UN #'S

Not Applicable

PACKING GROUP

Not Applicable

HAZARD CLASS

Not Applicable

Handling

Warning.Toxic. Potential Carcinogenic. Do not use this compound if you are not fully trained or are unaware of the hazards involved. Wear gloves/mask when handling product. Avoid contact by all means of exposure. Avoid contact with skin, eyes or clothing. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Use with local exhaust ventilation.

Citations

CELL BIOLOGY: Sororin cooperates with the acetyltransferase Eco2 to ensure DNA replication-dependent sister chromatid cohesion Andrea L. Lafont, Jianhua Song, and Susannah Rankin PNAS, Nov 2010; 107: 20364 - 20369.

Citations_Hyperlink

http://www.pnas.org/cgi/reprint/107/47/20364

Certificate of Analysis 1

A-1026, K1158H.pdf

Certificate of Analysis 2

A-1026, J1034E.pdf

Certificate of Analysis 3

A-1026, J1034D.pdf

Reviews

Safety

GHS PICTOGRAMS
Handling	Warning.Toxic. Potential Carcinogenic. Do not use this compound if you are not fully trained or are unaware of the hazards involved. Wear gloves/mask when handling product. Avoid contact by all means of exposure. Avoid contact with skin, eyes or clothing. Do not breathe dust/fume/gas/mist/vapors/spray. Do not eat, drink or smoke when using this product. Use with local exhaust ventilation.
UN #'S	Not Applicable
Packing Group	Not Applicable

Citations

Citations	Análisis de la señalización por Ret: caracterización de la cascada molecular B-Raf/IKKS y regulación de Ret por Sprouty1
	A balance between elongation and trimming regulates telomere stability in stem cells
	Arabidopsis POT1 associates with the telomerase RNP and is required for telomere maintenance
	Sororin cooperates with the acetyltransferase Eco2 to ensure DNA replication-dependent sister chromatid cohesion
	Early and late steps in telomere overhang processing in normal human cells: the position of the final RNA primer drives telomere shortening
	Endoplasmic reticulum stress-induced apoptosis requires bax for commitment and Apaf-1 for execution in primary neurons
	Cytosine arabinoside rapidly activates Bax-dependent apoptosis and a delayed Bax-independent death pathway in sympathetic neurons
	The limited role of NH2-terminal c-Jun phosphorylation in neuronal apoptosis: identification of the nuclear pore complex as a potential target of the JNK pathway
	Secreted herpes simplex virus-2 glycoprotein G modifies NGF-TrkA signaling to attract free nerve endings to the site of infection
	Inhibition of the c-Jun N-terminal kinase signaling pathway by the mixed lineage kinase inhibitor CEP-1347 (KT7515) preserves metabolism and growth of trophic factor-deprived neurons
	Secreted herpes simplex virus-2 glycoprotein G alters thermal pain sensitivity by modifying NGF effects on TRPV1
	Exposure to the polybrominated diphenyl ether mixture DE-71 damages the nigrostriatal dopamine system: role of dopamine handling in neurotoxicity
	Cohesin acetylation promotes sister chromatid cohesion only in association with the replication machinery
	RET modulates cell adhesion via its cleavage by caspase in sympathetic neurons
	Human mismatch repair: reconstitution of a nick-directed bidirectional reaction
	JNK-independent activation of c-Jun during neuronal apoptosis induced by multiple DNA-damaging agents
	BH3-only Bcl-2 family members are coordinately regulated by the JNK pathway and require Bax to induce apoptosis in neurons
	The activation loop phosphorylation of protein kinase D is an early marker of neuronal DNA damage
	Identification of JNK-dependent and -independent components of cerebellar granule neuron apoptosis
	Mixed-lineage kinase inhibitors require the activation of Trk receptors to maintain long-term neuronal trophism and survival
	Formaldehyde-induced genome instability is suppressed by an XPF-dependent pathway
	Alterations to the circuitry of the frontal cortex following exposure to the polybrominated diphenyl ether mixture, DE-71
	Temporal differences in DNA replication during the S phase using single fiber analysis of normal human fibroblasts and glioblastoma T98G cells
	DNA replication in early S phase pauses near newly activated origins
	Developmental exposure to the organochlorine insecticide endosulfan damages the nigrostriatal dopamine system in male offspring
	Early development and axis specification in the sea anemone Nematostella vectensis
	Selective damage to dopaminergic transporters following exposure to the brominated flame retardant, HBCDD
	Developmental exposure to the organochlorine insecticide endosulfan alters expression of proteins associated with neurotransmission in the frontal cortex
	DNA damage checkpoint responses in the S phase of synchronized diploid human fibroblasts
	Alteration to Dopaminergic Synapses Following Exposure to Perfluorooctane Sulfonate (PFOS), in Vitro and in Vivo
	Herpes simplex virus glycoprotein G enhances chemotaxis through modi!cation of plasma membrane microdomains and receptor tra
	TrkA COOH-terminal tail: its relevance on receptor stability and signaling for cell differentiation and survival
	Defining Multiple Steps in Human Telomere End Processing
	Telomere -associated proteins in Arabidopsis thaliana
	Zellteilungsregulation meristematischer Wurzelzellen (Tabak BY-2) durch Phytohormone und Zucker
	Genetic analysis of the Replication Protein A large subunit family in Arabidopsis reveals unique and overlapping roles in DNA repair, meiosis and DNA replication
	Genetic and evolutionary analysis of plant replication protein A 1 (RPA1)
	Fragile X Mental Retardation Protein regulates R-loop formation and prevents global chromosome fragility