Protein Solubilizers aka Protein Biodetergents

November 9, 2011 Categories AG Scientific Blog

Protein Solubilizers aka Protein Biodetergents

Chemically and Biologically Pure Protein Biodetergents offered by AG Scientific, Inc.

Feature

Benefit

Very low contaminating Aldehyde and Peroxide concentration < 0.1 mM To maximize yields of biologically active proteins
Extremely low conductivity < 5 µmhos Prevents problems associated with interfering salts and metal ions
10% solution in sterile water for injection High purity and ease of dispensing of diluted solutions
Pre-scored 5 ml Amber Ampoules Small package size with amber ampoules to maximize shelf life
Sterile, U.S.P Grade To maintain biological purity, Improved stability.
Aseptically manufactured and filled, Large Scale GMP  capabilities For Research use through therapeutic Production capabilities

Non-Ionic Biodetergents

Non-ionic detergents contain uncharged, hydrophilic head groups that consist of eithermicellepolyoxyethylene moieties as in TWEEN and TRITON or glycosidic groups as in octyl glucoside and dodecyl maltoside. In general, non-ionic detergents are better suited for breaking lipid-lipid and lipid-protein interactions than protein-protein interactions. Hence, they are considered non-denaturant and are widely used in the isolation of membrane proteins in their biologically active form.

The use and performance of a detergent is dependent on a number of general properties such as hydrophobicity, CMC, aggregation number, charge etc…(TABLE-1 : Properties of Non-Ionic Detergents).

Several investigators have reported problems associated with aldehyde and peroxide impurities in polyoxyethylene detergents 5,6,8. There has also been documented evidence of support of microbial growth and toxic metabolite production in non sterilized aqueous solutions of these detergents.

Our product line of Highly purified, Sterile, 10% aqueous solutions of detergents should offer a definite advantage to those working with sensitive membrane-bound proteins.

Stability/Storage/Handling: This product is sterile & stable for 2+ years as supplied. Store in a cool dry place. Protect from light.

 

TABLE -1: Properties of Non-Ionic Detergents

Product

Cat.No.

M.W.a

CMCb

(mM)

Agg.

No.

Avg.

Micellar

Wt.

HLB

No.

Cloud

Point

Protein Solubilizer 80(10% Soln of Tween 80)

P-1498

1310

0.012

58

76,000

15

Protein Solubilizer 20(10% Soln of Tween 20)

P-1502

1228

0.059

16.7

Protein Solubilizer X-100(10% Soln of Triton X-100)

P-1500

625

0.2-0.9

140

80,000

13

64ºC

Protein Solubilizer X-114(10% Soln of Triton X-114)

P-1496

427

0.35

12.4

22ºC

Protein Solubilizer 35(10% Soln of Brij-35)

P-1504

1198

0.09

40

48,000

16.9

Protein Solubilizer 40(10% Soln of NP-40)

P-1505

603

0.05-0.3

13.1

80ºC

Protein Solubilizer Set-1(5 Sample Set: 2X5 ml each)

P-1503

a) Average molecular weights for detergents composed of mixtures of chain lengths; b) Temperature: 20-25oC

 

References

Banerjee, P., et al. (1993). Differential solubilization of membrane lipids by detergents. Arch. Biochem. Biophys. 305, 68.

Neugebauer, J. M. (1990). Detergents: An overview. Methods. Enzymol. 182, 239.

Furth, A. H., et al. (1984). Separating detergents from proteins. Methods. Enzymol. 104, 318.

Hjelmeland, L. M., and Chrambach, A. (1984). Solubilization of functional membrane proteins. Methods. Enzymol. 104, 305.

Ashani, Y. and Catravas, G. (1980). Highly reactive impurities in Triton X-100 and Brij 35: partial characterization and removal. Anal. Biochem. 109, 55-62.

Chang, H. W. and Bock E. (1980). Pitfalls in the use of commercial nonionic detergents for the solubilization of integral membrane proteins: sulfhydryl oxidizing contaminants and their elimination. Anal. Biochem. 104, 112-117.

Helenius, A., McCaslin, D. R., Fries, E., and Tanford, C. (1979). Properties of detergents. Methods. Enzymol. 56, 734-749.

Lever, M. (1977). Peroxides in detergents as interfering factors in biochemical analysis. Anal. Biochem. 83, 274.

Slinde, E., and Flatmark, T. (1976). Effect of the hydrophile-lipophile balance of non-ionic detergents on the solubilization of biological membranes and their integral b-type cytochromes. Biochim. Biophys. Acta. 455, 796.

Helenius, A. and Simons, K. (1975). Solubilization of membranes by detergents. Biochim. Biophys. Acta. 415, 29-79

Triton is a registered trademark of Union Carbide.

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