Side by Side comparison, DTT VS TCEP, Preferred Reducing Reagents

November 17, 2011 Categories AG Scientific Blog

Side by Side comparison, DTT VS TCEP, Preferred Reducing Reagents

Description: TCEP. HCl has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent, more hydrophilic, and more resistant to oxidation in air. Near complete reduction of disulfide bonds. DTT is a protective agent for reducing –S-S TO SH groups. Used as a strong reducing agent for proteins and enzymes.
Alternative name/s: Tris(2-Carboxyethyl) phosphine.hydrochloride DTT is also known as Dithiothreitol or originally as Cleland’s Reagent after discover, W.W. Cleland, University of Wisconsin.
CAS # 51805-45-9 3483-12-3
M.W. 286.6 154.25
Molecular Formula: C9H16O6PCl C4H10O2S2
Chemical structure:  T-1180, TCEP Chemical Structure  C-1029, DTT Chemical Structure
Appearance ODORLESS, white crystals Slight sulfur smell, white crystalline powder
Solubility: 310 g/L 50g/L
Stability I: Non-volatile. Effective at room temperature. When refrigerated 2-8ºC, it is stable up to 3 years.
Stability II: Stable at room temperature. Stable in aqueous, acidic & basic solutions. We can ensure stability ≤ 72 hours without significant degradation or oxidation.
Degradation: TCEP is not very stable in phosphate buffers, especially at neutral pH. Since TCEP is charged in solution, it is not compatible for use in isoelectric focusing. Yes, when stressed at 30ºC DTTdemonstrates degradation starting after 3 days and increasing rapidly after 5 days. Sensitive to nickel.
Effective pH Effective at lower/wider pH. Effective pH range is 1.5 to 8.5. When dissolved in water resulting pH is ~2.5. Yes, the reducing power is limited to pH values >7.Redox potential is -0.33 V at pH 7.
Packaging requirements? Package under inert gas, protect from moisture. Package under inert gas, protect from moisture.
Removal required? TCEP removal is not required prior to most applications (e.g. histidine-tagged protein purification, maleimideconjugations). DTT is removed by filtration (for the solid catalyst) or by chromatography (for the liquid form).
Uses: Popular for Protein applications, SDS-PAGE, mass spectrometry applications, TCEP does not reduce metals used in immobilized metal affinity chromatography. TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis, histidine-tagged protein purification, maleimideconjugations. DTT is as a reducing or “deprotecting” agent for thiolated DNA. Electrophoresis.


16 thoughts on “Side by Side comparison, DTT VS TCEP, Preferred Reducing Reagents”

  1. Magnificent goods from you. I have remember your stuff previous
    to and you’re simply extremely excellent. I really like what you have obtained right here, certainly like what you are saying and the way during which you are
    saying it. You make it entertaining and you
    still take care of to stay it wise. I can not wait
    to learn much more from you. This is really a wonderful website.

    1. Thank you! We appreciate the positive feedback. We are currently hosted on the Nexcess server. We work hard to optimize our load time.

      Wishing you the best in building your site.

      Idelle Delapena
      Marketing Director
      AG Scientific

  2. Hello! Do you know if they make any plugins to safeguard
    against hackers? I’m kinda paranoid about losing everything I’ve worked hard
    on. Any tips?

    1. Hello –

      We work with our web development team to prevent spam. I would work directly with your team on this issue. Web developers have extensive experience in this topic.

      Idelle Delapena
      Marketing Director
      AG Scientific

    1. Hello – we have an RSS feed button on our website as well as a newsletter subscribe box at the bottom of our website.

      Thank you for reading and choosing to subscribe.

      Idelle Delapena
      Marketing Director
      AG Scientific

  3. Does your site have a contact page? I’m having
    problems locating it but, I’d like to shoot you an e-mail.
    I’ve got some recommendations for your blog you might be interested in hearing.
    Either way, great site and I look forward to seeing it improve
    over time.

  4. I’ve been surfing online more than three hours today, yet
    I never found any interesting article like yours.
    It is pretty worth enough for me. Personally, if all webmasters
    and bloggers made good content as you did, the net will
    be much more useful than ever before.

Leave a Reply

Your email address will not be published. Required fields are marked *