Chymostatin (Protease Inhibitor) - FAQs

Microbial product chymostatin was discovered by H. Umezawaet al.v in 1970 by testing the anti-chymotrypsin activity of culture filtrates. Since the amino acid analysis of different samples gave variable ratios of phenylalanine, leucine, valine and isoleucine, chymostatin was considered to be a mixture of similar peptides with minor differences. Attempts to separate the components were unsuccessful.

Fig 1: Chemical structure of chymostatin

This product is sparingly soluble in all solvents except acetic acid and dimethylformamide. It gave a positive test with triphenyltetrazolium chloride but was negative to ninhydrin, suggesting the presence of a reducing function and the absence of a free amino group.

Chymostatin is a bioactive peptide protease inhibitor that contains chymostatin A which is a major form, B, and C forms.Chymostatin is a strong inhibitor of many proteases, including chymotrypsin, papain, chymotrypsin-like serine proteinases, chymases, and lysosomal cysteine proteinases such as cathepsins A,B,C, B, H, and L.

Chymostatin (20uM) decreased protein breakdown by 20-40% in leg muscles from normal rodents and also in denervated and dystrophic muscles. The related inhibitors pepstatin, bestatin, and elastatinal did not decrease protein breakdown.

Chymostatin did not decrease protein synthesis and thus probably retards proteolysis by a specific effect on cell proteinase(s). In addition, chymostatin, but not leupeptin, inhibits the chymotrypsin-like proteinase apparent in muscle homogenates. In muscles depleted of most of this activity by treatment with the mast-cell-degranulating agent 48/80, chymostatin still decreased protein breakdown. Therefore inhibition of this alkaline activity probably does not account for the decrease in protein breakdown. These results are consistent with a lysosomal site of action for chymostatin.

Frequently Asked Questions

  • QUESTION: WHAT IS Bio-structure of Chymostatin?

ANSWER: (S)-1-Carboxy-2-Phenylethyl]-Carbamoyl-a-[2-Iminohexahydro-4(S)-Pyrimidyl]-(S)-Glycyl-X-Phenylalaninal

  • QUESTION: Is there any alternative name?

ANSWER: Yes, Chemicon (Millipore).

  • QUESTION: What are the basic chemical data?

ANSWER: 
Chemical Formula: C25H30N6O5.X  (X value varies by lot)

CAS Number:9706-44-2

Appearance:White to Off White powder

Molecular Mass: chymostatin A MW = 607.7, chymostatin B MW = 593.7, chymostatin C MW = 607.7

Working conditions: 10-100μM.

  • QUESTION: What is Chymostatin's solubility?

ANSWER: DMSO (20mg/ml); Glacial Acetic Acid (10mg/ml)

  • QUESTION: What about Storage conditions?

ANSWER: Maintain lyophilized material at -20ºC for up to one year. Store reconstituted product in aliquots at -20ºC for up to 3-4 weeks. Do not thaw and refreeze.

Source: Tatsuta, K., et al. 1973. J. Antibiot. 26: 625-646. PMID, Effects of Chymostatin and other Proteinase Inhibitors on Protein Breakdown and Proteolytic Activities in Muscle, Peter LIBBY and Alfred L. GOLDBERG Department ofPhysiology, Harvard Medical School, Boston, MA 02115, U.S.A, Wikipedia.

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  1. Chymostatin
    Chymostatin
    C-1469

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