Properties of specific Protease, Phosphatase, Serine Protease Inhibitors

Protease inhibitor cocktails are a unique series of enzyme inhibitors that knock out specific proteases to avoid peptide bond hydrolysis and subsequent protein destruction. Protease inhibitors are vital to scientists engaged in proteomic research, protein expression, characterization, protein modification, protein profiling, and quantitative measurement of proteins to prevent destructive protein degradation.

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Phosphatases play an important role in many signal transduction pathways, as the addition or removal of a phosphate group can activate or de-activate an enzyme, or allow a particular protein-protein interaction. Phosphatase inhibitors vary in function, targeting specific unwanted phosphatases to prevent their activity. Combinations of inhibitors in cocktail form can selectively target particular contaminants. A.G. Scientific maintains a selection of standard inhibitor cocktails in stock, and retains the ability to prepare custom formulations.

Inhibitor Target for Inhibition Specifics
AEBSF Serine proteases such as thrombin, plasmin, kallikrein, chymotrypsin, and trypsin

 

Irreversible inhibitor, lower toxicity than PMSF or DFP.
Leupeptin Serine and cysteine proteases. Often used for inhibition of plasmin, trypsin, papain, kallikrein, and cathepsin B.

 

Reversible inhibitor
Antipain Papain and trypsin, and to a smaller extent plasmin

 

More specific to papain and trypsin than leupeptin and 100-fold more potent than elastatinal.
Aprotinin Kallikrein, trypsin, chymotrypsin, and various serie proteases

 

Competitive, reversible inhibitor
E-64 Cysteine proteases

 

Irreversible inhibitor
Pepstatin A Aspartic proteases. Pepsin, cathespin D, rennin, chymosin, and HIV proteases.

 

Reversible inhibitor
Bestatin Aminopeptidase Binds to cell surfaces. Also activates macrophages and T lymphocytes.

 

Phosphoramidon Endothelin converting enzyme

IC50 = 0.68μM

Also inhibits neutral endopeptidase 24.11 and neprilysin 2

 

Calyculin A Protein phosphatase 1 and 2A Also potent non-phorbol tumor promoter
Microcystin LR Protein phosphatase 1 and 2A Potent inhibitor

 

Cantharidin Protein phosphatase 2A Also inhibits protein phosphatase 1 at higher concentrations. Useful in inhibition of PP-1 and PP-2A during purification of phosphorylated proteins.

 

Bromotetramisole Alkaline phosphatases

 

Elastinal Elastase-like serine proteases produced by actinomycetes Competitive inhibitor. Also inhibits PMSF-stimulated degredation of histone H1 and HMG proteins and α2-macroglobulin-protease complexes.

 

GGACK

(click for your free GGack Brochure)

Urokinase (IC50 < 1 μM) and Factor Xa Irreversible inhibitor

 

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  1. AEBSF HCl | CAS 30827-99-7 | irreversible serine protease inhibitor | AG Scientific Inc
    AEBSF HCl
    A-1018

    Starting at $49.99

  2. Calyculin A, Discodermia calyx
    Calyculin A, Discodermia calyx
    C-1031

    Starting at $152.99

  3. Phosphoramidon
    Phosphoramidon
    P-1101

    Starting at $61.81

  4. Bestatin HCl | CAS 65391-42-6 | Inhibitors | AG Scientific, Inc.
    Bestatin HCl
    B-1075

    Starting at $34.99

  5. Cantharidin
    Cantharidin
    C-1412

    Starting at $70.36

  6. Leupeptin | CAS 103476-89-7 | Protease Inhibitor | AG Scientific, Inc.
    Leupeptin, Hemisulfate
    L-1165

    Starting at $30.00

  7. Aprotinin | CAS 9087-70-1 | Protease Trypsin Inhibitor | AG Sci, Inc.
    Aprotinin, bovine lung
    A-1420

    Starting at $44.67

  8. Antipain | CAS 37682-72-7 | Papain & Trypsin Inhibitor | AG Scientific
    Antipain Dihydrochloride
    A-1448

    Starting at $39.99

  9. Pepstatin A | CAS 26305-03-3 | Aspartic Protease Inhibitor | AG Scientific, Inc.
    Pepstatin A
    P-1519

    Starting at $36.90

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9 Items

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