blasticidin_s_gene_selection_antibiotic_for_mammaliam_cell_linesBlasticidin S is an antibiotic used by scientists in bio-research to select cells modified by genetic engineering. It inhibits the growth of a wide range of prokaryotic and eukaryotic by interfering with protein synthesis cells.

Blasticidin S allows the selection and maintenance of cells expressing the blasticidin-resistance gene. Blasticidin S is a peptidyl nucleoside antibiotic isolated from the culture broth of Streptomyces griseochromogenes. It specifically inhibits protein synthesis in both prokaryotes and eukaryotes through inhibition of peptide-bond formation in the ribosomal machinery. It is used to select transfected cells carrying bsr or BSD resistance genes. Blasticidin S specifically inhibits protein synthesis in both prokaryotes and eukaryotes through inhibition of peptide-bond formation in the ribosomal machinery.

How do you determine Blasticidin sensitivity?

  • Plate cells at approximately 25% confluency. Prepare a set of 6 plates. Allow cells to adhere overnight.
  • The next day, substitute culture medium with medium containing varying concentrations of Blasticidin ( e.g. 0,2,4,6,8,10 µg / mL Blasticidin)
  • Replenish the selective media every 3-4 days, and observe the percentage of surviving cells.
  • Determine the appropriate concentration of Blasticidin that kills the cells within 10-14 days after addition of the antibiotic.

What mammalian cell lines work with Blasticidin S?

Cell Type Selection Concentration ( µg/ml)
10T1/2 cells ~10 µg/ml
293 HEK 3-10 µg/ml
293T Lα ~10 ug/ml
A2780 ~5 µg/ml
A549 2.5 to 10 ug/mL
ASK 2.5-10 µg/ml
B16 3-10 µg/ml
CHO 5-10 µg/ml
COS-1 3-10 µg/ml
COLO 320DM ~10 µg/ml
CSII-EF-RfA ~ 5 µg/ml
Cell Type Selection Concentration (µg/ml)
CV1-P 2.5-6 µg/ml
D. discoideum coupled w/ Klebsiella aerogenes ~ 10 µg/ml
DH1 ~10 µg/ml
DF-1 10 ug/ml
EBV ~5 µg/ml
Giardia cells ~75 ug/ml
HAV 1-3 µg/ml
HEK293T 2.5 to 10 ug/mL
HeLa-S3 2.5 to 10 ug/mL
HeLa 2.5 to 10 ug/mL
HIV 10 µg/ml
HMEC-1 2.5-6 µg/ml
HMX44A ~10 µg/ml
HT1080 2.5 to 10 ug/mL
FIV 10 µg/ml
iPS ~ 10 ug/ml
JH10 ~10 µg/ml
IMCD ~5 ug/ml
L929 2.5 to 10 ug/mL
PC1.0 10-30 µg/ml
MHOM/IL/80/Friedlin 10 µg/ml
N2a 2.5 to 10 ug/mL
NIH-3T3 2.5 to 10 ug/mL
Nannochloropsis sp (algae) ~5 ug/mL
Plasmodium falciparum 2.5-5.0 ug/ml
SHK-1 2.5-10 µg/ml
S2 Drosophila ~ 5 µg/ml
S. cerevisiae ~25 µg/ml
TRE5-A ~5 µg/ml
T3-L1 2.5-10 µg/ml
thraustochytrids 100 ug/ml
TO 2.5-10 µg/ml
U2OS 2.5 to 10 ug/mL

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    Blasticidin S HCl
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  2. Blasticidin S HCl Solution in HEPES buffer (10 mg/mL)
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