Î²-lactamase-broad spectrum has broad scope of activity against both penicillins (penicillinase or Beta I activity), up to 5th generation cephalosporins (cephalosporinase or Beta II activity), as well as, the Carbapenems:Â Ertapenem, Imipenem,Â Meropenem.
Î²-LACTAMASE-BS is a freeze-dried product containing buffer salts and zinc. Î²-LACTAMASE-BS has a broader spectrum activity of complete inactivation of a broad range of cephalosporins and penicillins.
|Amdinocillin, Amoxicillin, Azlocillin, Benzylpenicillin, Carbenicillin, Cloxacillin, Flucloxacillin, Methicillin, Mezlocillin, Nafcillin, Oxacillin, Penicillin, Piperacillin, Ticarcillin.|
Î²-LACTAMASE-BS has been demonstrated to inactivate the followingÂ CEPHALOSPORINS:
1STÂ GENERATION:Â Cefadroxil, Cefalexin,Cefaloridine, Cefazolin.
2NDÂ GENERATION:Â Cefaclor, Cefamandole, Cefmetazole, Cefonicid, Cefotetan, Cefoxitin, Cefprozil, Cefuroxime.
5THÂ GENERATION: Ceftobiprole
Î²-LACTAMASE-BS has been demonstrated to inactivate the following
CARBAPENEMS:Â Ertapenem, Imipenem, Meropenum.
Î²-Lactamase-BroadÂ SpectrumÂ Applications:
1. Testing Blood Culture Sterility
|Blood cultures are routinely prepared in order to test for bacterial infection. False negative results might be obtained where the blood sample contains antibiotics. Incorporation of Î²-lactamase in the culture medium will overcome this problem when cephalosporins /penicillins are present.|
2. Testing for Contamination of Drugs by Antibiotics
3. Environmental Monitoring of Antibiotic Manufacturing Areas
|Contact plates, settle plates and air monitoring systems for testing of aseptic conditions in antibiotic manufacturing facilities need to be manufactured with agar medium for neutralization of antibiotic. This is achieved by the addition of Penicillinase or Î²-Lactamase to the medium. Through this, any antibiotic residues are hydrolyzed and microbial contamination can be detected.|
4. Sterility Testing of Bulk Antibiotics
|US Pharmacopeia (USP) Chapter 71 and EP Section 2.6 describe sterility testing of bulk antibiotics, which should be shown to be free from microbial contamination. The testing requires the removal of significant amounts of active antibiotic from solution by combined filtration and the use of penicillinase or Î²-Lactamase, The resulting solution is tested for the (lack of) growth of microbes. USP specifies that the amount of Penicillinase or Î²-Lactamase used in this removal process should be verified using a microbial challenge solution in a control sample.|
|In light of the overuse of Î²-lactam antibiotics and resulting resistance, new and novel broader spectrum Î²-lactamase (L-2467) is an essential tool. For environmental and sterility monitoring of antibiotic manufacturing facilities under FDA (21 CFR211.176 and CFR436.20), broader spectrum Î²-Lactamase is now a requirement.|
|FULL INACTIVATION: of a broad range of Î²-Lactams including cephalosporin: Cefepime, Cefoxitin, Cefradine and Ceftaxidime. Other commercially available enzymes do not degrade as efficiently or as comprehensively.|
|HIGHER STABILITY: Retains <90% of its activity after 4 hours at 50Â°C, which means no need to compensate for loss of enzyme activity during media preparation for applications utilizing sterile plates.|
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