A.G. Scientific, Inc. is pleased to offer Anisomycin (Product # A-1049), isolated and purified from Streptomyces griseolus.
Anisomycin is a bacterial antibiotic which inhibits protein synthesis by binding to 60S ribosomal subunits and blocking peptide bond formation. This prevents protein elongation and causing polysome stabilization. This action of Anisomycin monitored the cells for chromosomal DNA degradation and apoptosis.1-5
Anisomycin has been widely used as an extremely potent activator of kinase cascades in mammalian cells, especially the stress-activated protein kinase (SAPK2/p38MAPK), kinase subtypes and p46/54JNK. 6-13
Anisomycin synergizes with growth factors and phorbol esters, and superinduces c-fos and c-jun by a number mechanisms, one of which is its ability to act as a potent signalling agonist, producing strong, prolonged activation of the same nuclear responses as epidermal growth factor or tetradecanoyl phorbol acetate.14
Anisomycin stimulated serine phosphorylation of IRS-1 and IRS-2, reduced their ability to interact with the insulin receptor and by that blocked the insulin-induced tyrosine phosphorylation of IRS proteins.15
Many of the most effective antibiotics used in medicine today are small molecules produced by fungi. The properties of an antibiotic are sometimes determined by its activity on the different regions on the bacterial ribosome. These molecules are effective by inhibiting bacterial protein synthesis. The antibiotic Anisomycin is an effective inhibitor of fungal and protozoal growth. Anisomycin uses an effective blocking protein that works by shutting down the peptidyl transferase reaction within ribosomes. The ribosomes of eucaryotic mitochondria (and chloroplasts) will often resemble those of bacteria in their sensitivity to inhibitors. Therefore, some antibiotics such as Anisomycin can have a deletrious effect on human mitochondria.
Anisomycin, also known as flagecidin is an antibiotic produced by Streptomyces griseolus which inhibits protein synthesis. Partial inhibition of DNA synthesis occurs at anisomycin concentrations that effect 95% inhibition of protein synthesis. Anisomycin can activate stress-activated protein kinases, MAP kinase and other signal transduction pathways. Anisomycin is inactive against bacteria.
Biological Role(s): DNA synthesis inhibitor Any substance that inhibits the synthesis of DNA. protein synthesis inhibitor A compound, usually an anti-bacterial agent or a toxin, which inhibits the synthesis of a protein. bacterial metabolite Any prokaryotic metabolite produced during a metabolic reaction in bacteria. antibiotic A substance that is biostatic or biocidal at low concentrations towards bacteria, yeasts, moulds, or other form of life, especially pathogenic or noxious organisms. Although the term was originally restricted to substances produced by microorganisms, its use was later expanded to include derivatives of such substances and it is now commonly used to include entirely synthetic compounds. (via heterocyclic antibiotic )
Application(s): antiparasitic agent A substance used to treat or prevent parasitic infections. antineoplastic agent A substance that inhibits or prevents the proliferation of neoplasms. antibiotic A substance that is biostatic or biocidal at low concentrations towards bacteria, yeasts, moulds, or other form of life, especially pathogenic or noxious organisms. Although the term was originally restricted to substances produced by microorganisms, its use was later expanded to include derivatives of such substances and it is now commonly used to include entirely synthetic compounds. (via heterocyclic antibiotic )
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