1. Breaking News (2016) - HIV Drug May Treat Alzheimer's Disease

    Breaking News (2016) - HIV Drug May Treat Alzheimer's Disease
    Scientists at Case Western University, under the support in part by the Public Health Service Grant, was able to map the allosteric site in Cholesterol Hydroxylase CYP46A1 for efavirenz, a drug used to treat HIV infections, that stimulates enzyme activity. What they Found CYP46A1 is an enzyme responsible for 80 percent of the cholesterol breakdown in the human brain. Efavirenz attaches to...
  2. Puromycin Trending Applications: shRNA Gene Silencing Protocol

    Puromycin Trending Applications: shRNA Gene Silencing Protocol
    Puromycin’s longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the CRISPR/Cas9 system. Here we discuss a protocol for shRNA Gene Silencing and CRISPR system transfection of which include puromycin. shRNA Plasmid DNA Mediated Inhibition of Gene Expression In a six well tissue culture plate, grow cells to a 50-70% confluency in antibiotic-free normal growth...
  3. Top Tips for a Better ELISA

    Top Tips for a Better ELISA
    What are your tips? Enzyme-linked immunosorbent assays (ELISA or EIA) are tests that detect and measure antibodies in your blood. It can determine the presence of antibodies, or proteins that are produced in response to harmful substances called antigens, that are related to certain infectious conditions. ELISA has been used to diagnose HIV, Lyme disease, pernicious anemia, rotavirus, squamous cell...
  4. FAQs About Aprotinin Serine Protease Inhibitor

    FAQs About Aprotinin Serine Protease Inhibitor
    Initially named "kallikrein inactivator”, Aprotinin was first isolated from cow parotid glands in 1930. As a pancreatic enzyme inhibitor, it was initially used in the treatment for acute pancreatitis, a condition in which the destruction of the gland by its own enzymes is thought to be part of the pathogenesis. Bovine pancreatic trypsin inhibitor (BPTI) is one of the most thoroughly studied proteins in...
  5. How to Cure Fear: Anisomycin, Propanolol, and the Brain

    How to Cure Fear: Anisomycin, Propanolol, and the Brain
    A phobia is a strong, irrational fear, often triggered by something that poses little to no actual threat. Phobias are considered an anxiety disorder and cripple the livelihood of many people, sometimes preventing them from performing a normal task without extreme discomfort. In 1999, neuroscientist Karim Nader tested a theory on the process of memory reconsolidation—a process in which a...

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