1. Puromycin Trending Applications: CRISPR Activation Plasmid Transfection

    Puromycin Trending Applications: CRISPR Activation Plasmid Transfection
    Puromycin, in conjunction with the CRISPR-CAS9 system, continues to be applied for genomic purposes. In this protocol, plasmid transfections utilizing these components will be described. PHASE 1. CRISPR Activation Plasmid Transient Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In a 6-well...
  2. Leupeptin and Hearing Loss (Leupeptin Applications)

    Leupeptin and Hearing Loss (Leupeptin Applications)
    Hearing loss, of which can be induced by either impulse noise or continuous noise, can induce temporary or permanent hearing loss dependent upon intensity and duration. Continuous noise is defined as noise that remains constant and stable over a certain period of time while impulse noises are intermittent and are composed of a rapid rise in the intensity of sound...
  3. Top 10 Women in Molecular Biology

    Top 10 Women in Molecular Biology
    When you put the helmet on, it doesn't matter if you are woman or man: your mission is to compete to win. -- Milka Duno Below are notable women in STEM that have greatly impacted the scientific community with their efforts. These are the top 10 women in molecular biology. Rosalind Franklin Since the age of 15, Rosalind Franklin wanted...
  4. Puromycin Trending Applications: Double Nickase Transfection

    Puromycin Trending Applications: Double Nickase Transfection
    Puromycin continues to be applied for genomic purposes. Continuing our puromycin series is a protocol on double nickase transfection. PHASE 1. Double Nickase Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In a 6-well tissue culture plate seed 1.5 x 105...
  5. THC Destroys Amyloid Beta in Alzheimer's (2016 Research)

    THC Destroys Amyloid Beta in Alzheimer's (2016 Research)
    Alzheimer’s disease, a brain disorder that causes memory loss and dementia, is one of the leading causes of death and affects more than five million Americans according to the National Institute of Health. With its incidence being projected to triple during the next 50 years, Alzheimer’s disease is an increasing problem with the elderly population. Amyloid Beta: What is it...
  6. Keys to Fighting Alzheimer's: 2016 Research

    Keys to Fighting Alzheimer's: 2016 Research
    (Image via NYtimes) Below are scientifically reported events that can help the general public and researchers understand the latest tools and methods to alleviate or combat the neurodegenerative disease that affects over 5 million Americans every year and 60% of 47.5 million people with dementia. Keep Your Cerebrovascular System Healthy Researchers reported those with arteriosclerosis and atherosclerosis had higher chances...
  7. Puromycin Trending Applications: CRISPR KO and HDR Plasmid Transfection

    Puromycin Trending Applications: CRISPR KO and HDR Plasmid Transfection
    Puromycin continues to be applied for genomic purposes. In this protocol, puromycin is utilized in KO and HDR CRISPR plasmid transfections. PHASE 1. CRISPR/Cas9 KO Plasmid and HDR Plasmid Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In a 6-well tissue...
  8. Top 10 Females in Biopharma

    "Leadership is about creating futures that are not a direct path," says the Broad Institute's Samantha Singer. "It's about being courageous and being willing to speak about and talk about futures that aren't obvious to other people. Take a stand for a future you can see, one you know can happen even when you can't see how." As for the future...
  9. Dementia Research Articles (2016)

    Dementia Research Articles (2016)
    A flurry of awesome research articles regarding Alzheimer's, Parkinson's, and other neurological disorders were published last week. Below are the top ones concerning dementia's relation to the cardiovascular system as well as a new bioengineered method of looking at the disease via protein-activation of neurotransmitters: Confirmed: There is No Risk of Contracting Dementia via Blood Transfusion What they found: The Karolinska Institutet...
  10. Puromycin Trending Applications: shRNA Lentiviral Particles Transduction

    Puromycin Trending Applications: shRNA Lentiviral Particles Transduction
    Puromycin continues to be applied for genomic purposes. In this protocol, puromycin is utilized in lentiviral transduction in shRNA particles. DAY 1 Plate target cells in a 12-well plate 24 hours prior to viral infection. Add 1 ml of complete optimal medium (with serum and antibiotics) and incubate cells overnight. The cells should be approximately 50% confluent on the day...

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