1. Attaining High DNA Yield from Sperm Cells using Proteinase K

    Attaining High DNA Yield from Sperm Cells using Proteinase K
    There are many techniques that can be used to isolate high quality and high molecular weight DNA from mammalian somatic cells. However, these techniques are ineffective for mammalian sperm. Unlike somatic cells, nearly all histones in sperm cells are replaced by protamines held together by disulfide bonds, which compacts the sperm nucleus, rendering it resistant to conventional lysis procedures. Sperm...

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