TCEP Reducing Agent: Frequently Asked Questions

AG Scientific is a leading supplier of TCEP HCl. Here we review some of the most frequently asked questions about this useful reducing agent:

What is TCEP?

This is a useful reducing agent with applications in molecular biology, biochemistry and microbiology

Are there alternative names for this product?

  • TCEP hydrochloride
  • Tris(2-carboxyethyl)phosphine hydrochloride

 

chemical structure of TCEP

Fig 1: chemical structure of TCEP hydrochloride

What is the chemical formula?

C9H15O6P • HCl

 

What is the molecular weight?

286.6 Da

 

What does this reagent look like?

AG Scientific's TCEP HCl is supplied as a white crystalline solid.

 

 

 

 

 

What is its solubility?

This reagent is soluble in water, forming a clear colorless solution.

Is this a hazardous product?

This reagent is corrosive. Always wear personal protective equipment when handling this reagent. It is an irritant to the eyes, skin, respiratory tract and digestive tract. Minimize dust generation and accumulation, avoid contact.

What is the mechanism of action?

Reducing reagents act by giving up electrons in a chemical reaction. When these substances donate electrons, they are considered to then be oxidized. The atom/substance where the electrons are sent to is called an oxidant. The reducing agent causes the oxidant to become reduced.

What are common applications of this product?

This reducing agent is commonly used to break disulfide bonds in proteins in preparation for analysis via gel electrophoresis. TCEP is a suitable substitute for DTT. This reagent is a useful analytical tool for quantification of sulfides, disulfides, hypochlorous acid, hypochlorite ions, iodine, and iodate ions.

How should this product be stored?

Solid form should be stored desiccated at -20°C. Protect from moisture.

ADDITIONAL READING

 

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