1. IPTG: Frequently Asked Questions and Protocols

    IPTG: Frequently Asked Questions and Protocols
    IPTG is a molecular biology reagent utilized in cloning experiments. This compound is used as a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lacoperon. Unlike allolactose, the IPTG sulfur (S) atom creates a chemical bond which is non-hydrolyzable by the cell, preventing the cell from "eating up" or degrading the inductant; therefore the IPTG concentration remains constant.
  2. IPTG Triggers the Transcription of the Lac Operon

    IPTG Triggers the Transcription of the Lac Operon
    Isopropyl β-D-1-thiogalactopyranoside, abbreviated IPTG, is a molecular biology reagent. This compound is used as a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon.
  3. 9 Tips Influencing Successful Puromycin Gene Transfection

    9 Tips Influencing Successful Puromycin Gene Transfection
    Puromycin is an aminonucleosidic antibiotic. Puromycin is a protein synthesis inhibitor by inhibiting translation. Puromycin’s main function is used in cell biology as a selective antibiotic agent in cell culture systems.

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