The polymerase chain reaction (PCR) technique, used to amplify or “copy” small segments of DNA, is highly sensitive - and that’s a good thing. PCR is a widely used and inexpensive technique used in medical diagnostics, forensic analysis of DNA, and genomic research. Unfortunately, this sensitivity leaves PCR vulnerable to nucleic acid contamination: even trace amounts of DNA can be...

Electrochemical Processes for Treating Water and Why it Matters Photo by Pok Rie, Pexels As the Earth’s population grows at an unprecedented rate, it is vital to ensure we invest in improving access and removing barriers to clean water, and that includes increasing the use of electrochemistry for water remediation. Scientists have determined that in line with our current population...

EDAC HCl Reducing Agent: Frequently Asked Questions AG Scientific is a leading supplier of EDAC HCl. Here we review some of the most frequently asked questions about this useful reducing agent: 1. What is EDAC HCl? EDAC Hydrochloride is a versatile modern reducing agent. It is an easily handled solid with high solubility in water and organic solvents such as dichloromethane...

TCEP Reducing Agent: Frequently Asked Questions AG Scientific is a leading supplier of TCEP HCl. Here we review some of the most frequently asked questions about this useful reducing agent: What is TCEP? This is a useful reducing agent with applications in molecular biology, biochemistry and microbiology Are there alternative names for this product? TCEP hydrochloride Tris(2-carboxyethyl)phosphine hydrochloride   Fig 1...

DTE Reducing Agent: Frequently Asked Questions AG Scientific is a leading supplier of DTE reducing agent. Here we review some of the most frequently asked questions about this useful chemical: What is DTE? DTE (formally 1,4-Dithioerythritol) is an epimer of DTT (dithiothreitol). This sugar is derived from the corresponding 4-carbon monosaccharide erythrose and contains sulfur. It is an epimer of...

5 Things to Consider When Choosing Biological Buffers According to ThoughtCo., biological buffers usually consist of a conjugate base and a weak acid or a conjugate acid and weak base. A buffer is a type of solution that helps maintain stable pH levels. Successful research is dependent on finding and using the right buffer. The following are five important aspects...

A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.

Sodium dodecyl sulfate (SDS or NaDS), sodiumlaurilsulfate or sodium laurel sulfate (SLS) is an organic compound with the formula CH3(CH2)11OSO3Na. It is an anionic surfactant used in many cleaning and hygiene products.

Dithiothreitol (DTT), also known as Cleland's reagent, is a small-molecule redox reagent. Its oxidized form is a disulfide-bonded 6-membered ring. DTT has an epimeric ('sister') compound, dithioerythritol (DTE).

TCEP. HCl has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent, more hydrophilic, and more resistant to oxidation in air. Near complete reduction of disulfide bonds. DTT is a protective agent for reducing –S-S TO SH groups. Used as a strong reducing agent for proteins and enzymes.