1. Part 1: Solving Mysteries with Trypsin and Proteinase K

    Part 1: Solving Mysteries with Trypsin and Proteinase K
    Identifying Human Remains Human remains are fascinating. They have many properties that can be used to identify a person: distinct scars, special features, dental x-rays, fingerprint comparisons, or physical facial characteristics. Forensic scientists attempt to identify human skeletal remains in situations like mass fatality incidents involving military personnel or others recovered from war, fires, explosions, and in criminal matters. However...
  2. Puromycin - Anti-Tumor Effect and Activity

    Puromycin - Anti-Tumor Effect and Activity
    Puromycin is an aminonucleoside antibiotic produced by the bacterium Streptomyces alboniger. It inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation.
  3. CK-666: Inhibitor of Actin-Related Proteins

    CK-666: Inhibitor of Actin-Related Proteins
    Actin is a family of globular multi-functional proteins that form microfilaments. Microfilaments, also called actin filaments, are filaments in the cytoplasm of eukaryotic cells that form part of the cytoskeleton. Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions...
  4. SMER28- Enhancer of Autophagy

    SMER28- Enhancer of Autophagy
    SMER28 is a small-molecule enhancer (SMER) of autophagy that increases autophagosome synthesis and enhances clearance of aggregate-prone substrates, including those relevant to Huntington's, Parkinson's, and Alzheimer's diseases. It is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant...
  5. Attaining High DNA Yield from Sperm Cells using Proteinase K

    Attaining High DNA Yield from Sperm Cells using Proteinase K
    There are many techniques that can be used to isolate high quality and high molecular weight DNA from mammalian somatic cells. However, these techniques are ineffective for mammalian sperm. Unlike somatic cells, nearly all histones in sperm cells are replaced by protamines held together by disulfide bonds, which compacts the sperm nucleus, rendering it resistant to conventional lysis procedures. Sperm...
  6. 8 Benefits of Choosing AGS as Your Proteinase K Supplier

    8 Benefits of Choosing AGS as Your Proteinase K Supplier
    In molecular biology Proteinase K (also protease K or endopeptidase K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).
  7. DNA isolation - Proteinase K method

    DNA Isolation from Tails: Each tail should be in a clean eppendorf tube. DNA Isolation from Tails: Add 500µl of tail lysis buffer containing Proteinase K (PK)to each tube. Incubate tail samples in 50-60C water bath overnight. Add 250µl saturated (6M) NaCl to each tube. ...
  8. Dithiothreitol aka Cleland's Reagent

    Dithiothreitol aka Cleland's Reagent
      Dithiothreitol or DTT is also known as Cleland’s Reagent. It contains two thiol groups and is commonly used to reduce disulfide bond in proteins. It can protect many thiol-containing biomolecules such as coenzyme A (Co-A), GSH (glutathione) from forming disulfide bonds. Not only does DTT scavenge the hydroxyl radical that attacks DNA and proteins, but also reacts with hydrogen...
  9. Neomycin Applications

    Neomycin Applications
    Neomycins are a family of broad-spectrum highly toxic antibiotics produced by Streptomyces fradiae. Neomycin usually exists as a complex composed of two stereoisomers, neomycin B and neomycin C, which are the two active components. A third component, neomycin A, which is a degradation product, is present in small quantities. Neomycin C was isolated by DUTCHER et al. Neomycin C is...
  10. Nonactin based biosensors to measure ammonia

    Nonactin based biosensors to measure ammonia
    Ammonia (NH3) is a poisonous and pungent-smelling colorless gas that makes the eyes water. Ammonia is readily soluble in water. The aqueous solution has an alkaline reaction and is known as ammonia water or tincture of ammonia. In conjunction with acid, ammonia forms salts that disassociate in water, to form ammonium ions (NH4 +) and the corresponding anions. In nature...

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