1. How to Use Proteases for Protein Proteolysis

    How to Use Protease/s for Protein Proteolysis Protein Proteolysis refers to the use of hydrochloric acid to destroy peptide bonds between the amino acids that make up a protein. When carried out with proteases (also known as proteinases), which are enzymes, the process is termed proteolysis. Depending on how efficient the enzyme is or what links it breaks, the proteolysis can be limited or unlimited. Proteolysis can begin either from one end of the chain of amino acids that comprise the protein or somewhere in the middle of it. Unlimited proteolysis produces individual amino acids while limited proteolysis produces short chains of polypeptides.
  2. Non-Ionic Protein Solubilizers (Non-Ionic Detergents)

    Non-ionic detergents contain uncharged, hydrophilic head groups that consist of eithermicelle polyoxyethylene moieties as in TWEEN and TRITON or glycosidic groups as in octyl glucoside and dodecyl maltoside. In general, non-ionic detergents are better suited for breaking lipid-lipid and lipid-protein interactions than protein-protein interactions.
  3. E-64, Cysteine Protease Inhibitor: Frequently Asked Questions

    E-64, Cysteine Protease Inhibitor: Frequently Asked Questions
    Irreversible, potent and highly selective inhibitor of cysteine proteases. Does not affect cysteine residues in other enzymes. Acts by forming a thioether bond with thiol of the active cysteine. E-64 will not inhibit serine proteases (except trypsin) inhibits activation-induced programmed cell death and restores defective immune responses in HIV+ donors. Specific active site titrant.
  4. Phosphatase Inhibitor Cocktails, Ready Made Formulations

    Invasive phosphatases can contaminate research samples and slow or halt research progress, rendering time-consuming and expensive work useless.
  5. 9 Tips for Selecting a Biological Detergent

    9 Tips for Selecting a Biological Detergent
    The method of detergent removal can be an important consideration. If dialysis is to be employed, a detergent with a high CMC is clearly preferred. Alternatively, if ion exchange chromatography is utilized, a non-ionic detergent (e.g. n-Octyl-β-D-Glucopyranoside, OG, O-1036 see structure,OCTYL-GLUCOSIDE DDM, D-1304) or a sulfobetaines.
  6. What are Biomarkers?

    Biomarkers are substances, structures, or processes that can be measured in biological samples such as urine, blood, or saliva. A biomarker is a measurable characteristic in a biological system that changes due to disease, exposure to chemicals, or exposure to organisms.
  7. 9 Tips Influencing Successful Puromycin Gene Transfection

    9 Tips Influencing Successful Puromycin Gene Transfection
    Puromycin is an aminonucleosidic antibiotic. Puromycin is a protein synthesis inhibitor by inhibiting translation. Puromycin’s main function is used in cell biology as a selective antibiotic agent in cell culture systems.
  8. FK506 (Tacrolimus): Frequently Asked Questions

    FK506 (Tacrolimus): Frequently Asked Questions
    A.G. Scientific is a leading supplier of the immunosuppressant, FK-506 commonly known as Tacrolimus. It is well known for its ability to prevent allograft rejection after transplantation.FK 506 resized 600 A.G.Scientific has long been established as a leading research supplier in the field of fermentation derived secondary metabolites.
  9. Neuroprotective Effects of Geneticin (G418) via Apoptosis

    Neuroprotective Effects of Geneticin (G418) via Apoptosis
    PURPOSE: Some antibiotics were known to exert neuroprotective effects in the animal model of hypoxic-ischemic (H-I) brain injury, but the mechanism is still unclear. A recent study reported that geneticin (G418), an aminoglycoside antibiotic, increased survival of human breast cancer cells by suppressing apoptosis. We investigated the neuroprotective effects of systemically administrated geneticin via anti-apoptosis following the H-I brain injury.

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