1. Blockade of Hsp90 by 17AAG antagonizes MDMX and synergizes with Nutlin to induce p53-mediated apoptosis in solid tumors

    Strategies to induce p53 activation in wtp53-retaining tumors carry high potential in cancer therapy. Nutlin, a potent highly selective MDM2 inhibitor, induces non-genotoxic p53 activation. Although Nutlin shows promise in promoting cell death in hematopoietic malignancies, a major roadblock is that most solid cancers do not undergo apoptosis but merely reversible growth arrest. p53 inhibition by unopposed MDMX is one...
  2. LY294002 - Cell Permeable Inhibitor of Phosphatidylinositol 3-Kinase (PI3K)

    LY294002 is a morpholine derivative of quercetin. It is a potent inhibitor of phosphoinositide 3-kinases (PI3Ks). PI3Ks (phosphatidylinositol 3-kinases) is an enzyme implicated in growth factor signal transduction by associating with receptor and nonreceptor tyrosine kinases, including the platelet-derived growth factor receptor. Inhibitors of PI3Ks could potentially give a better understanding of the function and regulatory mechanisms of the enzyme...
  3. Vascular Stem Cells and Tumor Angiogenesis - Pt. 2

    HPCs AND THEIR PROGENY The role of hematopoietic cells in tumor angiogenesis has recently received great attention. HPC from the bone marrow emigrate to the blood and tissues and differentiate to form cells of the innate and adaptive immune system. Tumor-infiltrating leukocytes can either protect against or promote tumor formation. The simplest example of the former is loss of immune...
  4. Vascular Stem Cells and Tumor Angiogenesis - Pt. 1

    Solid tumors are complex “organs” of cancer cells and a heterogeneous population of hematopoietic cells, mesenchymal cells, and endothelial cells. The cancer stem cell model proposes that tumor growth and progression is driven by rare populations of cancer stem cells
  5. Chymostatin (Protease Inhibitor) - Frequently Asked Questions

    Microbial product, chymostatin was discovered by H. Umezawaet al.v in 1970 by testing the anti-chymotrypsin activity of culture filtrates. Since the amino acid analysis of different samples gave variable ratios of phenylalanine, leucine, valine and isoleucine, chymostatin was considered to be a mixture of similar peptides with minor differences. Attempts to separate the components were unsuccessful.
  6. Proteomics: Pathways and Biomarkers

    Proteomics Proteomics is about measuring proteins, and ideally, about measuring many of them. Not surprisingly, given the universal importance of proteins in biological systems and the variety of their properties when employed as machines, signals, structural materials, and food, proteomics has enabled real progress in many areas of biology while falling short in others. Fortunately, we are beginning to understand...
  7. Proteomics: addressing the challenges of multiple myeloma

    Multiple myeloma (MM) is a malignancy of terminally differentiated B-lymphocytes that accounts for ∼13% of all hematologic cancers. Despite a wealth of knowledge describing the molecular biology of MM as well as significant advances in therapeutics, this disease remains incurable.
  8. Cost Effective Solution to the Precission Protease

    Human rhinovirus 3C protease (HRV 3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission SiteTM. It cleaves between Gln and Gly.
  9. How to Use Proteases for Protein Proteolysis

    How to Use Protease/s for Protein Proteolysis Protein Proteolysis refers to the use of hydrochloric acid to destroy peptide bonds between the amino acids that make up a protein. When carried out with proteases (also known as proteinases), which are enzymes, the process is termed proteolysis. Depending on how efficient the enzyme is or what links it breaks, the proteolysis can be limited or unlimited. Proteolysis can begin either from one end of the chain of amino acids that comprise the protein or somewhere in the middle of it. Unlimited proteolysis produces individual amino acids while limited proteolysis produces short chains of polypeptides.
  10. Non-Ionic Protein Solubilizers (Non-Ionic Detergents)

    Non-ionic detergents contain uncharged, hydrophilic head groups that consist of eithermicelle polyoxyethylene moieties as in TWEEN and TRITON or glycosidic groups as in octyl glucoside and dodecyl maltoside. In general, non-ionic detergents are better suited for breaking lipid-lipid and lipid-protein interactions than protein-protein interactions.

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