1. What is Thapsigargin? Frequently Asked Questions

    What is Thapsigargin? Frequently Asked Questions
    Thapsigargin is derived from the plant Thapsia garganica that produces tumor promoters. This effect is a result of emptying the intracellular calcium stores, which leads to a chain of events that causes apoptosis. Mechanism of Action Chemical structure of Thapsigargin Thapsigargin is an inhibitor of sarco endoplasmic reticulum Ca2 ATPase (SERCA). It pumps calcium ions from the cytoplasm into the lumen...
  2. CHAPS Detergent: Protocols and Frequently Asked Questions

    CHAPS Detergent: Protocols and Frequently Asked Questions
    A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.
  3. RNase A: Frequently Asked Questions

    RNase A: Frequently Asked Questions
    Introduction to RNase Ribonucleases (RNases) are a large group of hydrolytic enzymes that degrade ribonucleic acid (RNA) molecules. These are nucleases that catalyze the breakdown of RNA into smaller components. They are a superfamily of enzymes which catalyze the degradation of RNA, operating at the levels of transcription and translation. 3D conformation of ribonuclease A enzyme These enzymes are present...
  4. Laboratory and Medicinal Uses for SDS Detergent

    Laboratory and Medicinal Uses for SDS Detergent
    Sodium dodecyl sulfate (SDS or NaDS), sodiumlaurilsulfate or sodium laurel sulfate (SLS) is an organic compound with the formula CH3(CH2)11OSO3Na. It is an anionic surfactant used in many cleaning and hygiene products.
  5. Hygromycin B: Most Frequently Asked Questions

    Hygromycin B: Most Frequently Asked Questions
    A.G.Scientific emerged as a leading manufacturer and importer of Hygromycin B, an antibiotic used for gene selection. Over the past 18 years we have learned a lot about this Aminoglycosides antibiotic. It was our success with a combination of fermentation & synthesis that has allowed us to build a customer base of researchers and catalog biochemical distributors worldwide. Questions on solution storage & what are the procedures to determine toxic concentration are common to all customers of Hygromycin. So here we listed the top questions we have received from our customers.
  6. Rapamycin: Most Commonly Asked Questions

    Rapamycin: Most Commonly Asked Questions
    Discovery of Rapamycin Rapamycin is a macrocyclic triene antibiotic that binds to the cytosolic protein FK-binding protein 12 (FKBP12) and inhibits mTOR pathway by directly binding to the mTOR Complex 1 (mTORC1). It is a potent immunosuppressant having a variety of uses both in vitro and in vivo and is used to prevent organ rejection after transplantation. Chemical structure of...
  7. IPTG Triggers the Transcription of the Lac Operon

    IPTG Triggers the Transcription of the Lac Operon
    Isopropyl β-D-1-thiogalactopyranoside, abbreviated IPTG, is a molecular biology reagent. This compound is used as a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon.
  8. Dithiothreitol (DTT) Applications You Must Know

    Dithiothreitol (DTT) Applications You Must Know
    Dithiothreitol (DTT), also known as Cleland's reagent, is a small-molecule redox reagent. Its oxidized form is a disulfide-bonded 6-membered ring. DTT has an epimeric ('sister') compound, dithioerythritol (DTE).
  9. Calcium Ionophore CA 1001: Applications in Medical Devices

    Calcium Ionophore CA 1001: Applications in Medical Devices
    As a chemical ionophore, CA 1001 (Calcium Ionophore I) has proven to be a high-quality biochemical. Its high selectivity for calcium ions makes it a great option for use in applications requiring the detection of calcium. CA 1001 can be used in calcium detection medical devices as well as biological membrane transport studies. What is Calcium Ionophore CA 1001? Calcium...
  10. TCEP HCl vs DTT: Preferred Reducing Agents

    TCEP HCl vs DTT: Preferred Reducing Agents
    TCEP. HCl has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent, more hydrophilic, and more resistant to oxidation in air. Near complete reduction of disulfide bonds. DTT is a protective agent for reducing –S-S TO SH groups. Used as a strong reducing agent for proteins and enzymes.
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