1. PMSF: Serine Protease Inhibitor Applications

    PMSF: Serine Protease Inhibitor Applications
    Understanding PMSF PMSF (phenylmethylsulfonyl fluoride) is an irreversible serine protease inhibitor used in various research and lab settings. The compound has a white powdery appearance at room temperature, with a melting point between 90-95°C. It functions by inhibiting serine proteases such as trypsin, chymotrypsin, and thrombin, and cysteine proteases like papain. The process involves the deactivation of enzymes that contain...
  2. Nanopore Electrochemistry: Single-Molecule Sensors

    Nanopore Electrochemistry: Single-Molecule Sensors
    A trend in modern scientific research over the past decade is using smaller & smaller methods of studying targets, like molecules. Independent, smaller devices, and less expensive methods allow more access for researchers across the board, regardless of their space and budget. One such analytical method is the creation of artificial nanopore sensors for research using nanopore electrochemistry. Capturing reactions...
  3. The SUnSET Assay: Powerful Proteomics

    The SUnSET Assay: Powerful Proteomics
    The SUnSET Assay: Powerful Proteomics Exciting advances in the study of protein synthesis and related health conditions are on the horizon! Research scientists have developed an improved process to quantify and understand the processes of protein synthesis in singular eukaryotic cells/cell populations. It’s called the surface sensing of translation, or SUnSET assay. It is a nonradioactive fluorescence-activated assay, which means...
  4. Pathology Research Using Mitophagy Modulators

    Pathology Research Using Mitophagy Modulators
    Pathology Research Using Mitophagy Modulators When you hear “small things with big impact”, do you immediately think of mitochondria and mitophagy modulators? Probably not! But these little organelles are champions of cell regulation.  Among their functions is regulating the energy of the cell as well as cell death. And just as mitochondria are responsible for cell function, mitophagy is responsible...
  5. Guanidine Thiocyanate in RNA Lysis Buffers and COVID-19 Research

    Guanidine Thiocyanate in RNA Lysis Buffers and COVID-19 Research
    Guanidine Thiocyanate in RNA Lysis Buffers and COVID-19 Research Guanidine thiocyanate chemical structure With COVID-19 ever present on our minds, viral research is being focused on now more than anything else. We at AG Scientific have remained open to meet the needs of the research community and are stocking chemical agents such as guanidine thiocyanate.  Read on to learn more...
  6. Tips for Selecting Biological Detergents

    Tips for Selecting Biological Detergents
    Tips for Selecting Biological Detergents Biological detergents are chemicals classified as surfactants, or surface-active molecules. Research scientists typically use these detergents in affecting the interactions within molecules of a biological sample being studied. These surfactants are used in biochemical research to target and manipulate the structure of membrane proteins without sacrificing their integrity or impacting their original function. Membrane proteins...
  7. EDAC HCl Reducing Agent: Frequently Asked Questions

    EDAC HCl Reducing Agent: Frequently Asked Questions
    EDAC HCl Reducing Agent: Frequently Asked Questions AG Scientific is a leading supplier of EDAC HCl. Here we review some of the most frequently asked questions about this useful reducing agent: 1. What is EDAC HCl? EDAC Hydrochloride is a versatile modern reducing agent. It is an easily handled solid with high solubility in water and organic solvents such as dichloromethane...
  8. TCEP Reducing Agent: Frequently Asked Questions

    TCEP Reducing Agent: Frequently Asked Questions
    TCEP Reducing Agent: Frequently Asked Questions AG Scientific is a leading supplier of TCEP HCl. Here we review some of the most frequently asked questions about this useful reducing agent: What is TCEP? This is a useful reducing agent with applications in molecular biology, biochemistry and microbiology Are there alternative names for this product? TCEP hydrochloride Tris(2-carboxyethyl)phosphine hydrochloride   Fig 1...
  9. DTE Reducing Agent: Frequently Asked Questions

    DTE Reducing Agent: Frequently Asked Questions
    DTE Reducing Agent: Frequently Asked Questions AG Scientific is a leading supplier of DTE reducing agent. Here we review some of the most frequently asked questions about this useful chemical: What is DTE? DTE (formally 1,4-Dithioerythritol) is an epimer of DTT (dithiothreitol). This sugar is derived from the corresponding 4-carbon monosaccharide erythrose and contains sulfur. It is an epimer of...
  10. 5 Things to Consider When Choosing Biological Buffers

    5 Things to Consider When Choosing Biological Buffers
    5 Things to Consider When Choosing Biological Buffers According to ThoughtCo., biological buffers usually consist of a conjugate base and a weak acid or a conjugate acid and weak base. A buffer is a type of solution that helps maintain stable pH levels. Successful research is dependent on finding and using the right buffer. The following are five important aspects...

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