1. CHAPS Detergent: Protocols and Frequently Asked Questions

    CHAPS Detergent: Protocols and Frequently Asked Questions
    A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.
  2. RNase A: Frequently Asked Questions

    RNase A: Frequently Asked Questions
    Introduction to RNase Ribonucleases (RNases) are a large group of hydrolytic enzymes that degrade ribonucleic acid (RNA) molecules. These are nucleases that catalyze the breakdown of RNA into smaller components. They are a superfamily of enzymes which catalyze the degradation of RNA, operating at the levels of transcription and translation. 3D conformation of ribonuclease A enzyme These enzymes are present...
  3. Laboratory and Medicinal Uses for SDS Detergent

    Laboratory and Medicinal Uses for SDS Detergent
    Sodium dodecyl sulfate (SDS or NaDS), sodiumlaurilsulfate or sodium laurel sulfate (SLS) is an organic compound with the formula CH3(CH2)11OSO3Na. It is an anionic surfactant used in many cleaning and hygiene products.
  4. Dithiothreitol (DTT) Applications You Must Know

    Dithiothreitol (DTT) Applications You Must Know
    Dithiothreitol (DTT), also known as Cleland's reagent, is a small-molecule redox reagent. Its oxidized form is a disulfide-bonded 6-membered ring. DTT has an epimeric ('sister') compound, dithioerythritol (DTE).
  5. TCEP HCl vs DTT: Preferred Reducing Agents

    TCEP HCl vs DTT: Preferred Reducing Agents
    TCEP. HCl has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent, more hydrophilic, and more resistant to oxidation in air. Near complete reduction of disulfide bonds. DTT is a protective agent for reducing –S-S TO SH groups. Used as a strong reducing agent for proteins and enzymes.
  6. Frequently Asked Questions About Proteinase K

    Frequently Asked Questions About Proteinase K
    In molecular biology Proteinase K (also protease K or endopeptidase K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album). Proteinase K is able to digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.
  7. Proteinase K - Bulk Packaging & Labeling Services

    Proteinase K - Bulk Packaging & Labeling Services
    AG Scientific is the established leader in Proteinase K bulk supply, catalog packaging, custom size vialing and private labeling services. We guarantee the following:
  8. Fighting HIV with Protease Inhibitors

    Fighting HIV with Protease Inhibitors
    HIV relies on the host cell it injects itself into to continue the cycle. By implicating road blocks in this process through protease inhibitors, there is a possibility of halting the replication and spread of the HIV virus. 7 Steps of the HIV Life Cycle: The first step of the HIV cycle is binding, when the virus first attaches to...
  9. 20 FACTS ABOUT PROTEASOMES & PROTEASOME INHIBITORS!

    1. Proteasome inhibitors are drugs that block the action of proteasomes, cellular complexes that break down proteins, like the p53 protein. Proteasome inhibitors are being studied in the treatment of cancer, especially multiple myeloma.
  10. DTT (Dithiothreitol): Frequently Asked Questions

    For the last 18 years, AG Scientific has been a leading supplier of DTT, dithiothreitol, historically known as Cleland’s Reagent. We offer a range of catalog sizes, as well as, multi-kilogram quantities for bulk applications. Additionally, we provide a full service of bottling, sterile formulations, custom packaging, as well as, comprehensive private labeling capabilities.

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