• Proteinase K Applications in Prion-Related Diseases 2018-11-10 11:01:49 Proteinase K degrades almost every protein and inactivates rapidly enzymatic activities under a broad range of conditions (SDS, Urea, pH 4 -12). The enzyme is used for the isolation of clean and native genomic DNA and RNA in order to guarantee PCR based detection. prions, prion, proteinase k
  • Beta-Lactamase Hybrid Proteins 2018-11-06 12:38:35 Introduction Beta-lactam antibiotics are used to treat a broad spectrum of Gram-positive and Gram-negative bacteria.   β-lactam antibiotics target the penicillin-binding proteins (PBPs) – a group of enzymes found attached in the cell membrane, which are involved in the cross-linking Beta Lactam Antibiotics
  • Attaining High DNA Yield from Sperm Cells using Proteinase K 2018-02-27 12:42:07 There are many techniques that can be used to isolate high quality and high molecular weight DNA from mammalian somatic cells. However, these techniques are ineffective for mammalian sperm. Unlike somatic cells, nearly all histones in sperm cells are replaced by protamines held together by disulfide proteinase k
  • Dithiothreitol aka Cleland’s Reagent 2017-10-02 09:57:11   Dithiothreitol or DTT is also known as Cleland’s Reagent. It contains two thiol groups and is commonly used to reduce disulfide bond in proteins. It can protect many thiol-containing biomolecules such as coenzyme A (Co-A), GSH (glutathione) from forming disulfide bonds. Not
  • n-Dodecyl-β-D-maltopyranoside 2017-06-09 15:06:39 Any scientist involved in proteomics or protein engineering, will stand by the fact that detergents are indispensable tools for isolation and solubilization of integral membrane proteins and protein complexes from biological membranes. For these studies, it is very important to have intact protein s
  • Detergents: Important Tools for Membrane Protein Purification 2017-05-30 09:23:08 Membrane Proteins Proteins control almost all functions of a cell. Any mutation in protein structure or any issue hampering protein functionality can lead to abnormal cell processes, which can in turn result in disease. Therefore it is very important to meticulously learn and get a better understand
  • New Tissue Atlas Shows Protein Distribution within the Human Body 2015-09-21 16:16:24 The preliminary mapping of The Human Protein Atlas project’s first detailed protein expression map was unveiled this week in Stockholm. This map, for the first time, includes a detailed display of systemic proteins, meaning of specific origin throughout the human body, with millions of high-r
  • Polyketide Enzymes Shuffle the Molecular Deck 2015-03-22 14:15:10 Our current knowledge… The applications of protein engineering can be found everywhere in biology. This field, known as proteomics, is a reliable means of producing novel molecules and has provided scientists a basic roadmap to understanding the essential properties of proteins. Therefore to
  • 3 Particularly Useful Biochemical Tools 2015-02-13 01:47:16 In addition to being a supplier of many important amino acids,enzymes and proteins, AG Scientific also sells many kinds of small molecules that have important and diverse biological functions.
  • Circulating Glioma Biomarkers: Agents of Detection 2014-10-17 13:36:42 High-grade gliomas represent one of the most aggressive and treatment-resistant types of human brain cancer, with a median survival rate of only 14.6 months for patients with grade IV glioma (glioblastoma) [1,2].