1. 5 Things to Consider When Choosing Biological Buffers

    5 Things to Consider When Choosing Biological Buffers
    5 Things to Consider When Choosing Biological Buffers According to ThoughtCo., biological buffers usually consist of a conjugate base and a weak acid or a conjugate acid and weak base. A buffer is a type of solution that helps maintain stable pH levels. Successful research is dependent on finding and using the right buffer. The following are five important aspects...
  2. CHAPS Detergent: Protocols and Frequently Asked Questions

    CHAPS Detergent: Protocols and Frequently Asked Questions
    A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.
  3. Dithiothreitol aka Cleland's Reagent

    Dithiothreitol aka Cleland's Reagent
      Dithiothreitol or DTT is also known as Cleland’s Reagent. It contains two thiol groups and is commonly used to reduce disulfide bond in proteins. It can protect many thiol-containing biomolecules such as coenzyme A (Co-A), GSH (glutathione) from forming disulfide bonds. Not only does DTT scavenge the hydroxyl radical that attacks DNA and proteins, but also reacts with hydrogen...
  4. Why should it be AG Scientific's HRV3C Protease?

    Why should it be AG Scientific's HRV3C Protease? Human rhinovirus 3C protease (HRV 3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly. The recombinant form of the HRV3C protease is a restriction grade protease that has robust activity at 4°C and high specific...

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