1. CHAPS Detergent: Protocols and Frequently Asked Questions

    CHAPS Detergent: Protocols and Frequently Asked Questions
    A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.
  2. 9 Tips for Selecting a Biological Detergent

    9 Tips for Selecting a Biological Detergent
    The method of detergent removal can be an important consideration. If dialysis is to be employed, a detergent with a high CMC is clearly preferred. Alternatively, if ion exchange chromatography is utilized, a non-ionic detergent (e.g. n-Octyl-β-D-Glucopyranoside, OG, O-1036 see structure,OCTYL-GLUCOSIDE DDM, D-1304) or a sulfobetaines.

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