1. Puromycin Trending Applications: shRNA Gene Silencing Protocol

    Puromycin Trending Applications: shRNA Gene Silencing Protocol
    Puromycin's longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the CRISPR/Cas9 system. Here we discuss a protocol for shRNA Gene Silencing and CRISPR system transfection of which include puromycin. shRNA Plasmid DNA Mediated Inhibition of Gene Expression In a six well tissue culture plate, grow cells to a 50-70% confluency in antibiotic-free normal...
  2. CRISPR-Cas9 Latest News: Infertility Research to Modify Embryos

    CRISPR-Cas9 Latest News: Infertility Research to Modify Embryos
      CRISPR-Cas9 technology has recently been implemented in Britain as the Human Fertilization and Embryology Authority granted its first license for the genetic modification of human embryos. These genetically modified embryos take part in the research of infertility, focusing on the source of miscarriages.   Dr. Kathy Niakan of the Francis Crick Institute plans to modify embryos utilizing the CRISPR-Cas9...
  3. The CRISPR Cas9 System: A War Against Antibiotic Resistance

    The CRISPR Cas9 System: A War Against Antibiotic Resistance
    Antibiotics (Hygromycin, Blasticidin, Neomycin, Geneticin/G-418, Puromycin) abuse is creating a whole new generation of antibiotic-resistant bacteria. As the tolerance to antibiotics grow, so does the battle against bacteria. The struggle continues to become a public issue. Humans are not the only ones trying to fight bacteria, however. Some viruses, the phages, have been doing it for millennia. Since the GAIN...

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