Puromycin, in conjunction with the CRISPR-CAS9 system, continues to be applied for genomic purposes. In this protocol, plasmid transfections utilizing these components will be described. PHASE 1. CRISPR Activation Plasmid Transient Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In...
Antibiotics (Hygromycin, Blasticidin, Neomycin, Geneticin/G-418, Puromycin) abuse is creating a whole new generation of antibiotic-resistant bacteria. As the tolerance to antibiotics grow, so does the battle against bacteria. The struggle continues to become a public issue. Humans are not the only ones trying to fight bacteria, however. Some viruses, the phages, have been doing it for millennia. Since the GAIN...
DNA (Deoxyribonucleic Acid) is often referred to as the blueprint for life. This is because DNA is made up of genes, which encode for the information required to produce functional proteins. describe the imageFor decades it was believed that only higher order, multi-cellular life possessed the cellular machinery necessary to provide its host with adaptive immunity. We now know that DNA is routinely edited in bacteria as part of their own adaptive immune response, which is made possible largely because of an enzyme called CRISPR â€“ pronounced â€˜crisperâ€™.