Puromycin's longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the CRISPR/Cas9 system. Here we discuss a protocol for shRNA Gene Silencing and CRISPR system transfection of which include puromycin. shRNA Plasmid DNA Mediated Inhibition of Gene Expression In a six well tissue culture plate, grow cells to a 50-70% confluency in antibiotic-free normal...
Antibiotics (Hygromycin, Blasticidin, Neomycin, Geneticin/G-418, Puromycin) abuse is creating a whole new generation of antibiotic-resistant bacteria. As the tolerance to antibiotics grow, so does the battle against bacteria. The struggle continues to become a public issue. Humans are not the only ones trying to fight bacteria, however. Some viruses, the phages, have been doing it for millennia. Since the GAIN...
DNA (Deoxyribonucleic Acid) is often referred to as the blueprint for life. This is because DNA is made up of genes, which encode for the information required to produce functional proteins. describe the imageFor decades it was believed that only higher order, multi-cellular life possessed the cellular machinery necessary to provide its host with adaptive immunity. We now know that DNA is routinely edited in bacteria as part of their own adaptive immune response, which is made possible largely because of an enzyme called CRISPR â€“ pronounced â€˜crisperâ€™.