1. Puromycin Trending Applications: CRISPR Activation Plasmid Transfection

    Puromycin Trending Applications: CRISPR Activation Plasmid Transfection
    Puromycin, in conjunction with the CRISPR-CAS9 system, continues to be applied for genomic purposes. In this protocol, plasmid transfections utilizing these components will be described. PHASE 1. CRISPR Activation Plasmid Transient Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In...
  2. Puromycin Trending Applications: CRISPR KO and HDR Plasmid Transfection

    Puromycin Trending Applications: CRISPR KO and HDR Plasmid Transfection
    Puromycin continues to be applied for genomic purposes. In this protocol, puromycin is utilized in KO and HDR CRISPR plasmid transfections. PHASE 1. CRISPR/Cas9 KO Plasmid and HDR Plasmid Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In a 6-well tissue...
  3. CRISPR-Cas9 Latest News: Infertility Research to Modify Embryos

    CRISPR-Cas9 Latest News: Infertility Research to Modify Embryos
      CRISPR-Cas9 technology has recently been implemented in Britain as the Human Fertilization and Embryology Authority granted its first license for the genetic modification of human embryos. These genetically modified embryos take part in the research of infertility, focusing on the source of miscarriages.   Dr. Kathy Niakan of the Francis Crick Institute plans to modify embryos utilizing the CRISPR-Cas9...
  4. The CRISPR Cas9 System: A War Against Antibiotic Resistance

    The CRISPR Cas9 System: A War Against Antibiotic Resistance
    Antibiotics (Hygromycin, Blasticidin, Neomycin, Geneticin/G-418, Puromycin) abuse is creating a whole new generation of antibiotic-resistant bacteria. As the tolerance to antibiotics grow, so does the battle against bacteria. The struggle continues to become a public issue. Humans are not the only ones trying to fight bacteria, however. Some viruses, the phages, have been doing it for millennia. Since the GAIN...

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