Digitonin (C56H92O29) is a steroidal, monodesmosidic saponin (a toxic chemical with a plant-based origin) derived from the foxglove plant species, Lady’s Glove; Digitalis pupurea. The compound appears as a white or off-white powder under room temperature, and scientists often apply it as a biological detergent due to its capacity to water-solubilize lipids. The compound naturally occurs as a non-ionic surfactant...
- Tips for Selecting Biological Detergents Biological detergents are chemicals classified as surfactants, or surface-active molecules. Research scientists typically use these detergents in affecting the interactions within molecules of a biological sample being studied. These surfactants are used in biochemical research to target and manipulate the structure of membrane proteins without sacrificing their integrity or impacting their original function. Membrane proteins...
- A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.
- Non-ionic detergents contain uncharged, hydrophilic head groups that consist of eithermicelle polyoxyethylene moieties as in TWEEN and TRITON or glycosidic groups as in octyl glucoside and dodecyl maltoside. In general, non-ionic detergents are better suited for breaking lipid-lipid and lipid-protein interactions than protein-protein interactions.
- The method of detergent removal can be an important consideration. If dialysis is to be employed, a detergent with a high CMC is clearly preferred. Alternatively, if ion exchange chromatography is utilized, a non-ionic detergent (e.g. n-Octyl-Î²-D-Glucopyranoside, OG, O-1036 see structure,OCTYL-GLUCOSIDE DDM, D-1304) or a sulfobetaines.