1. CHAPS Detergent: Protocols and Frequently Asked Questions

    CHAPS Detergent: Protocols and Frequently Asked Questions
    A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.
  2. Laboratory and Medicinal Uses for SDS Detergent

    Laboratory and Medicinal Uses for SDS Detergent
    Sodium dodecyl sulfate (SDS or NaDS), sodiumlaurilsulfate or sodium laurel sulfate (SLS) is an organic compound with the formula CH3(CH2)11OSO3Na. It is an anionic surfactant used in many cleaning and hygiene products.
  3. Non-Ionic Protein Solubilizers (Non-Ionic Detergents)

    Non-ionic detergents contain uncharged, hydrophilic head groups that consist of eithermicelle polyoxyethylene moieties as in TWEEN and TRITON or glycosidic groups as in octyl glucoside and dodecyl maltoside. In general, non-ionic detergents are better suited for breaking lipid-lipid and lipid-protein interactions than protein-protein interactions.
  4. 9 Tips for Selecting a Biological Detergent

    9 Tips for Selecting a Biological Detergent
    The method of detergent removal can be an important consideration. If dialysis is to be employed, a detergent with a high CMC is clearly preferred. Alternatively, if ion exchange chromatography is utilized, a non-ionic detergent (e.g. n-Octyl-β-D-Glucopyranoside, OG, O-1036 see structure,OCTYL-GLUCOSIDE DDM, D-1304) or a sulfobetaines.

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