1. Sources of DNA Contamination in the Laboratory

    Sources of DNA Contamination in the Laboratory
    The polymerase chain reaction (PCR) technique, used to amplify or “copy” small segments of DNA, is highly sensitive - and that’s a good thing. PCR is a widely used and inexpensive technique used in medical diagnostics, forensic analysis of DNA, and genomic research. Unfortunately, this sensitivity leaves PCR vulnerable to nucleic acid contamination: even trace amounts of DNA can be...
  2. Guanidine Thiocyanate in RNA Lysis Buffers and COVID-19 Research

    Guanidine Thiocyanate in RNA Lysis Buffers and COVID-19 Research
    With COVID-19 ever present on our minds, viral research is being focused on now more than anything else. We at AG Scientific have remained open to meet the needs of the research community and are stocking chemical agents such as guanidine thiocyanate.  Read on to learn more about this chemical and how it can aid your lab research. What Is...
  3. RNase A: Frequently Asked Questions

    RNase A: Frequently Asked Questions
    Introduction to RNase Ribonucleases (RNases) are a large group of hydrolytic enzymes that degrade ribonucleic acid (RNA) molecules. These are nucleases that catalyze the breakdown of RNA into smaller components. They are a superfamily of enzymes which catalyze the degradation of RNA, operating at the levels of transcription and translation. 3D conformation of ribonuclease A enzyme These enzymes are present...
  4. Frequently Asked Questions About Proteinase K

    Frequently Asked Questions About Proteinase K
    In molecular biology Proteinase K (also protease K or endopeptidase K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album). Proteinase K is able to digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.

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