1. Detergents: Important Tools for Membrane Protein Purification

    Detergents: Important Tools for Membrane Protein Purification
    Membrane Proteins Proteins control almost all functions of a cell. Any mutation in protein structure or any issue hampering protein functionality can lead to abnormal cell processes, which can in turn result in disease. Therefore it is very important to meticulously learn and get a better understanding of proteins in order to find therapies for diseases plaguing humanity. Integral membrane...
  2. Puromycin Trending Applications: CRISPR Activation Plasmid Transfection

    Puromycin Trending Applications: CRISPR Activation Plasmid Transfection
    Puromycin, in conjunction with the CRISPR-CAS9 system, continues to be applied for genomic purposes. In this protocol, plasmid transfections utilizing these components will be described. PHASE 1. CRISPR Activation Plasmid Transient Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In...
  3. Puromycin Trending Applications: Double Nickase Transfection

    Puromycin Trending Applications: Double Nickase Transfection
    Puromycin continues to be applied for genomic purposes. Continuing our puromycin series is a protocol on double nickase transfection. PHASE 1. Double Nickase Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In a 6-well tissue culture plate seed 1.5 x 105...
  4. Puromycin Trending Applications: CRISPR KO and HDR Plasmid Transfection

    Puromycin Trending Applications: CRISPR KO and HDR Plasmid Transfection
    Puromycin continues to be applied for genomic purposes. In this protocol, puromycin is utilized in KO and HDR CRISPR plasmid transfections. PHASE 1. CRISPR/Cas9 KO Plasmid and HDR Plasmid Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In a 6-well tissue...
  5. Puromycin Trending Applications: shRNA Lentiviral Particles Transduction

    Puromycin Trending Applications: shRNA Lentiviral Particles Transduction
    Puromycin continues to be applied for genomic purposes. In this protocol, puromycin is utilized in lentiviral transduction in shRNA particles. DAY 1 Plate target cells in a 12-well plate 24 hours prior to viral infection. Add 1 ml of complete optimal medium (with serum and antibiotics) and incubate cells overnight. The cells should be approximately 50% confluent on the day...
  6. Puromycin Trending Applications: shRNA Gene Silencing Protocol

    Puromycin Trending Applications: shRNA Gene Silencing Protocol
    Puromycin's longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the CRISPR/Cas9 system. Here we discuss a protocol for shRNA Gene Silencing and CRISPR system transfection of which include puromycin. shRNA Plasmid DNA Mediated Inhibition of Gene Expression In a six well tissue culture plate, grow cells to a 50-70% confluency in antibiotic-free normal...
  7. New Study to Explore Cause of Sudden Deaths

    Sudden unexplained death among individuals is a long-standing mystery among forensic scientists. On June 26th in San Diego, Jason Lappies took a seat on his sofa to watch a World Cup Soccer match before his shift at work started. When his housemate returned later that day however, Jason remained lying on the sofa in the same position as that morning...

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