1. IPTG: Frequently Asked Questions and Protocols

    IPTG: Frequently Asked Questions and Protocols
    IPTG is a molecular biology reagent utilized in cloning experiments. This compound is used as a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lacoperon. Unlike allolactose, the IPTG sulfur (S) atom creates a chemical bond which is non-hydrolyzable by the cell, preventing the cell from "eating up" or degrading the inductant; therefore the IPTG concentration remains constant.
  2. IPTG Triggers the Transcription of the Lac Operon

    IPTG Triggers the Transcription of the Lac Operon
    Isopropyl β-D-1-thiogalactopyranoside, abbreviated IPTG, is a molecular biology reagent. This compound is used as a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon.
  3. Top 7 FAQs About Beta-Lactamase

    Top 7 FAQs About Beta-Lactamase
    Top 7 FAQs About Beta-Lactamase As a core structure in many antibiotics, the beta-lactam ring is a cyclic amide. Beta-lactam antibiotics almost universally inhibit bacterial cell wall synthesis and thus induce the destruction of bacterial cells. Bacteria have evolved an enzyme that combats this antibiotic and increases resistance to death induced by antibiotic means: the beta-lactamase enzyme. Beta-lactamase enzymes can...
  4. IPTG in New Research: sRNAS & Alternative Inducers (2016)

    IPTG in New Research: sRNAS & Alternative Inducers (2016)
    IPTG mimics allolactase, a lac operon inducer, and is commonly used in scientific research to create proteins. It does not break down as allolactase would, keeping protein creation in steady production depending on IPTG concentration. Below are recent scientific articles that highlight some IPTG's applications in current research, and some of the future of its use. Novel sRNAS in E. coli Who: Geunu...
  5. IPTG in Synthetic Biology: Top 3 Review Papers

    IPTG in Synthetic Biology: Top 3 Review Papers
    Need IPTG? Well look no further! Learn more about it in our brochure! Improving the Lac System for Synthetic Biology Pallavi Penumetcha, Kin Lau, Xiao Zhu, Kelly Davis, Todd T. Eckdahl, A. Malcolm Campbell Figure 1. The lac system is the most widely used regulatory system in synthetic biology. Synthetic biology is an emerging field of study blending biology with...

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