IPTG is a molecular biology reagent utilized in cloning experiments. This compound is used as a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lacoperon. Unlike allolactose, the IPTG sulfur (S) atom creates a chemical bond which is non-hydrolyzable by the cell, preventing the cell from "eating up" or degrading the inductant; therefore the IPTG concentration remains constant.
IPTG mimics allolactase, a lac operon inducer, and is commonly used in scientific research to create proteins.Â ItÂ does not break down as allolactase would, keeping protein creation in steady production depending on IPTG concentration. Below are recent scientific articles that highlight some IPTG's applications in current research, and some of the future of its use. Novel sRNAS in E. coli Who:Â Geunu...