Reagents in Protein Purification The key to successful protein purification is choosing the correct process and the correct media and reagents. Although purification can sometimes be a simple one-step precipitation, multiple step processes are more frequently required to attain the desired purity.
The method of detergent removal can be an important consideration. If dialysis is to be employed, a detergent with a high CMC is clearly preferred. Alternatively, if ion exchange chromatography is utilized, a non-ionic detergent (e.g. n-Octyl-Î²-D-Glucopyranoside, OG, O-1036 see structure,OCTYL-GLUCOSIDE DDM, D-1304) or a sulfobetaines.