1. Selecting Reagents for Protein Purification

    Reagents in Protein Purification The key to successful protein purification is choosing the correct process and the correct media and reagents. Although purification can sometimes be a simple one-step precipitation, multiple step processes are more frequently required to attain the desired purity.
  2. 9 critical tips when selecting a Biological Detergent

    The method of detergent removal can be an important consideration. If dialysis is to be employed, a detergent with a high CMC is clearly preferred. Alternatively, if ion exchange chromatography is utilized, a non-ionic detergent (e.g. n-Octyl-β-D-Glucopyranoside, OG, O-1036 see structure,OCTYL-GLUCOSIDE DDM, D-1304) or a sulfobetaines.

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