1. Puromycin Trending Applications: CRISPR Activation Plasmid Transfection

    Puromycin Trending Applications: CRISPR Activation Plasmid Transfection
    Puromycin, in conjunction with the CRISPR-CAS9 system, continues to be applied for genomic purposes. In this protocol, plasmid transfections utilizing these components will be described. PHASE 1. CRISPR Activation Plasmid Transient Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In...
  2. Puromycin Trending Applications: Double Nickase Transfection

    Puromycin Trending Applications: Double Nickase Transfection
    Puromycin continues to be applied for genomic purposes. Continuing our puromycin series is a protocol on double nickase transfection. PHASE 1. Double Nickase Transfection This protocol is recommended for a single well from a 6-well tissue culture plate. Adjust cell and reagent amounts proportionately for wells or dishes of different sizes. In a 6-well tissue culture plate seed 1.5 x 105...

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