1. Frequently Asked Questions About Proteinase K

    Frequently Asked Questions About Proteinase K
    In molecular biology Proteinase K (also protease K or endopeptidase K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album). Proteinase K is able to digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.
  2. AG Scientific Offers Bulk Proteinase K Packaging & Labeling Services

    AG Scientific Offers Bulk Proteinase K Packaging & Labeling Services
    AG Scientific is the established leader in Proteinase K bulk supply, catalog packaging, custom size vialing and private labeling services. We guarantee the following:
  3. Part 2: Solving Mysteries with Trypsin and Proteinase K

    Part 2: Solving Mysteries with Trypsin and Proteinase K
    In Part 1, we discussed using trypsin on bone specimens as a cleaning alternative to sanding in preparation for DNA extraction. In this second part of the series, we cover the details of attaining a higher yield of DNA using proteinase K. Proteinase K for Higher DNA Yield from Bone Specimens DNA in bone is located in the osteocytes (Hochmeister et...
  4. Part 1: Solving Mysteries with Trypsin and Proteinase K

    Part 1: Solving Mysteries with Trypsin and Proteinase K
    Identifying Human Remains Human remains are fascinating. They have many properties that can be used to identify a person: distinct scars, special features, dental x-rays, fingerprint comparisons, or physical facial characteristics. Forensic scientists attempt to identify human skeletal remains in situations like mass fatality incidents involving military personnel or others recovered from war, fires, explosions, and in criminal matters. However...
  5. Attaining High DNA Yield from Sperm Cells using Proteinase K

    Attaining High DNA Yield from Sperm Cells using Proteinase K
    There are many techniques that can be used to isolate high quality and high molecular weight DNA from mammalian somatic cells. However, these techniques are ineffective for mammalian sperm. Unlike somatic cells, nearly all histones in sperm cells are replaced by protamines held together by disulfide bonds, which compacts the sperm nucleus, rendering it resistant to conventional lysis procedures. Sperm...

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