Puromycin continues to be applied for genomic purposes. In this protocol, puromycin is utilized in lentiviral transduction in shRNA particles. DAY 1 Plate target cells in a 12-well plate 24 hours prior to viral infection. Add 1 ml of complete optimal medium (with serum and antibiotics) and incubate cells overnight. The cells should be approximately 50% confluent on the day...
- Puromycin is an antibiotic researchers can use when working with cell cultures when they need a selective agent, a compound that will kill all cells without resistance genes, leaving only specific targets behind. This antibiotic is available through laboratory suppliers and is suitable for research purposes only.