1. Puromycin Trending Applications: shRNA Lentiviral Particles Transduction

    Puromycin Trending Applications: shRNA Lentiviral Particles Transduction
    Puromycin continues to be applied for genomic purposes. In this protocol, puromycin is utilized in lentiviral transduction in shRNA particles. DAY 1 Plate target cells in a 12-well plate 24 hours prior to viral infection. Add 1 ml of complete optimal medium (with serum and antibiotics) and incubate cells overnight. The cells should be approximately 50% confluent on the day...
  2. Puromycin Trending Applications: shRNA Gene Silencing Protocol

    Puromycin Trending Applications: shRNA Gene Silencing Protocol
    Puromycin'sĀ longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the CRISPR/Cas9 system. Here we discuss a protocol for shRNA Gene Silencing and CRISPR system transfection of which include puromycin. shRNA Plasmid DNA Mediated Inhibition of Gene Expression In a six well tissue culture plate, grow cells to a 50-70% confluency in antibiotic-free normal...

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