MECHANISM OF ACTION: epirubicidin, anthracycline, antibiotics, anticancerThe mechanism of action of epirubicin appears to be related to its ability to bind to nucleic acids. It forms a complex with DNA by intercalation between base pairs, resulting in inhibition of DNA and RNA synthesis. Intercalation also triggers DNA cleavage by topoisomerase II, resulting in cytocidal activity.
- Doxorubicin; trade name Adriamycin; also known as hydroxydaunorubicin, is a drug used in cancer chemotherapy. It is an anthracycline antibiotic, closely related to the natural product daunomycin, and like all anthracyclines, it works by intercalating DNA, while most serious adverse effect being life-threatening heart damage.
- Puromycin N-acetyl transferase gene (pac), of which the gene product catalyzes antibiotic puromycin, an effective inhibitor of protein synthesis, has been widely used as a dominant selection marker in embryonic stem (ES) cell-mediated transgenesis.
- Hygromycin B is an antibiotic used by scientists in bio-research to select cells modified by genetic engineering. It inhibits the growth of a wide range of prokaryotic and eukaryotic cells by interfering with protein synthesis. Hygromycin B allows the selection and maintenance of cells expressing the hygromycin-resistance gene.
- Tumor necrosis factorâ€“related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent because it induces apoptosis in cancer cells but not in normal cells.
- Combined treatment with a proteasome inhibitor and tumor necrosis factorâ€“related apoptosis-inducing ligand (TRAIL) is a promising strategy for cancer therapy.
- The PI3K/AKT/mTOR pathway is central to cell growth and survival, cell cycle regulation, and programmed cell death.Aberrant activation of this signaling cascade is linked to several disease states, and thus many components of the pathway are attractive targets for therapeutic intervention.
- Cancer Treated by Purifying Proteins Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The starting material is usually a biological tissue or a microbial culture. The various steps in the purification...