Bluo-Gal

SKU
X-1016
  • CAS:97753-82-7
  • Formula:C14H16BrNO6
  • MW:374.2 Da
  • Appearance:White powder
  • Purity:≥99% (TLC)
Product Name Qty
Bluo-GAL
100 mg
$44.88
Bluo-GAL
1 g
$147.90

Description

Bluo-Gal (5-Bromo-3-indolyl β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase (LacZ), used to determine the presence or absence of a cloned DNA inserted in bacteria growing on agar plates.

When it is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. The precipitate is insoluble in alcohol and xylenes, thus it is suitable for immunoblotting and immunocytochemical assays.

Upon microscopic examination under polarized light, the precipitated crystals emit a strong signal consisting of yellow reflected light. Because of this property, Bluo-Gal has more potent sensitivity of the staining method for β-galactosidase and has optimal morphological resolution.

Bluo-Gal can be used as an alternative of X-Gal. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

 

Applications

• Confirmation of cloned DNA insert in bacteria

• Lac gene detection systems

• Detection of β-gal activity by electron microscopy

• Immunoblotting

• Immunocytochemical assays

• Used in conjunction with IPTG for blue-white screening

 

References

• “Improved in situ β-galactosidase staining for histological analysis of transgenic mice”

• “lacZ Transgenic mice to monitor gene expression in embryo and adult”

 

Not for human therapeutic use or for medicinal purposes. For research applications only.

 

More Information
Alternate Name/Synonyms
Blue-gal; 5-Bromo-3-indolyl β-D-galactopyranoside
SKU
X-1016
CAS #
97753-82-7
Chemical Name
(2S,3R,4S,5R,6R)-2-[(5-bromo-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
Chemical Formula
C14H16BrNO6
Molecular Weight
374.2 Da
Appearance
White powder
Purity
≥99% (TLC)
Solubility
Clear and colorless solution (Sol. 2%, DMF)
Storage Temp
-20°C, desiccated
USE
Bluo-Gal is a substrate for beta-galactosidase that is converted, by the enzyme, into an insoluble indigoblue chromophore. It is also used in Lac gene detection systems, in immunoblotting and immunocytochemical assays.
MDL NUMBER
MFCD00063691
CHEMACX
X1076984-7
INCHI
InChI=1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
SMILES
c1cc2c(cc1Br)c(c[nH]2)O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O
Handling
Use only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
GHS PICTOGRAMS
HandlingUse only in area provided with appropriate exhaust ventilation. Keep away from heat and source of ignition. Empty containers pose a fire risk, evaporate residue under fume hood. Ground all equipment containing material. Do not breathe dust.
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