Applications:

Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca²⁺ on separate Ca²⁺-binding site. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme substrate calmoldulin-Ca²⁺ complexes are particularly stable.

Technical specifications

• Ligand: Calmodulin (Bovine)
• Matrix: Separopore® 4B (agarose beads, 4 %)
• Particle size range: 52 – 165 µm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Matrix activation: Cyanogen bromide
• Ligand density: ~ 1 mg calmodulin / ml drained gel
• Matrix attachment: Amino
• Matrix spacer: 1 atom
• pH stability: 4 – 9
• Storage: 2 – 8 °C
• Chemical stability: Stable to all commonly used aqueous solutions
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal