Calmodulin-SH, Wheat (Triticum aestivum)

SKU
C-1421
  • Appearance:Lyophilized
  • Purity:98% by SDS-PAGE
Product Name Qty
Calmodulin-SH, Wheat (Triticum aestivum)
100 ug
$5.13

Wheat germ (Triticum aestivum) calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26). In contrast to mammalian CaMs, which lack cysteine, this preparation is ideal for applications requiring both calmodulin activation and residue specific tagging. The single SH moiety at Cys26 can be labeled using a variety of sulfhydryl (SH) reactive protein-modification reagents such as fluorescent maleimide derivatives. Additionally, since wheat germ CaM contains only a single tyrosine residue (Tyr138) verses two tyrosine residues found in mammalian CaM, Tyr138 serves as a mono-specific C-terminal region labeling site. Using Cys26 and Tyr138 allows wheat germ CaM to be independently derivatized at both N- and C-terminal regions within the molecule for structural, proteomic biomarker discovery and protein-protein interaction studies. Wheat germ CaM also has no tryptophan resulting in a distinctive absorbance spectrum when compared to mammalian CaM sequences.

More Information
Alternate Name/Synonyms
CaMTag™ DuoSite
SKU
C-1421
CAS #
N/A
Chemical Name
CAMTAG™ DUOSITE
Chemical Formula
N/A
Appearance
Lyophilized
Purity
98% by SDS-PAGE
Solubility
1 ml/mL in H2O, CaM is completely clear in solution
Preparation
Stained with Coomassie blue having a single band at ~17 kDa
Storage Temp
-20°C
Merck Index
N/A
Handling
Store in Tightly Sealed Vial
Citations
BIOPHYSICS AND COMPUTATIONAL BIOLOGY: Subunit architecture of general transcription factor TFIIH Brian J. Gibbons, Edward J. Brignole, Maia Azubel, Kenji Murakami, Neil R. Voss, David A. Bushnell, Francisco J. Asturias, and Roger D. Kornberg PNAS, Feb 2012; 109: 1949 - 1954.
Citations_Hyperlink
http://www.pnas.org/cgi/reprint/109/6/1949
Certificate of Analysis 1
Certificate of Analysis 2
Certificate of Analysis 3
Handling Store in Tightly Sealed Vial
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