Calpain-1, Porcine Erythrocytes

SKU
C-1569
  • MW:112000
  • Appearance:Liquid
Product Name Qty
Calpain-1, Porcine Erythrocytes
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Native calpain-1 from porcine erythrocytes. The two major isoforms, calpain I (-form) and calpain II (m-form), differ in their calcium requirement for activation. Calpain I requires only micromolar amounts of calcium (EC = 2 M), while calpain II requires millimolar amounts (EC = 1 mM). Calpains are heterodimers of 80 kDa and 30 kDa subunits. The 80 kDa unit has the catalytic site and is unique to each isozyme. The 30 kDa unit is a regulatory subunit and common to both calpain I and calpain II. The 80 kDa unit consists of four domains (I-IV). The 30 kDa unit has two domains (V and VI). Domain I is partially removed during autolysis. Domain II is the protease domain. Domain III exhibits a homology with typical calmodulin binding proteins and interacts with calcium binding domains (IV and VI) and frees domain II for protease activity. Domain IV is a calcium binding domain. Domain V contains a hydrophobic region and is essential for calpain interaction with membranes. Domain VI is a calcium binding domain Specific Activity: 1200 units/mg protein. Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorogenic. 1 caseinolytic unit = 9 fluorogenic units
More Information
Alternate Name/Synonyms
μ-Calpain
SKU
C-1569
Molecular Weight
112000
Appearance
Liquid
Storage Temp
-80°C
THERAPEUTIC AREA
Not Available
USE
Not Available
MDL NUMBER
Not Available
CHEMACX
Not Applicable
INCHI
Not Applicable
SMILES
Not Applicable
Peptide sequence
Not Applicable
RTECS
Not Applicable
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Avoid freeze/thaw. Following initial thaw, aliquot and freeze (-70°C). The enzyme will lose activity upon storage at 4°C.
GHS PICTOGRAMS
HandlingAvoid freeze/thaw. Following initial thaw, aliquot and freeze (-70°C). The enzyme will lose activity upon storage at 4°C.
UN #'SNot Applicable
Packing GroupNot Applicable
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