Red fluorescent protein. A major goal of fluorescent protein development has become the construction of a red-emitting derivative that equals or exceeds the advanced properties of enhanced green fluorescent protein. Among the advantages of a suitable red fluorescent protein are the potential compatibility with existing confocal and widefield microscopes (and their filter sets), along with an increased capacity to image entire animals, which are significantly more transparent to red light. Because the construction of red-shifted mutants from theAequorea victoria jellyfish green fluorescent protein beyond the yellow spectral region has proven largely unsuccessful, investigators have turned their search to the tropical reef corals.
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FREE REVIEW ARTICLES
- Red Fluorescent Protein pH Biosensor to Detect Concentrative Nucleoside Transport, Danielle E. Johnson, Hui-wang Ai, et al. Red Fluorescent Protein pH Biosensor to Detect Concentrative Nucleoside Transport. J. Biol. Chem, 2009; 284: 20499 - 20511.
- Red Fluorescent Protein Responsible for Pigmentation in Trematode-Infected Porites compressa Tissues, Caroline V. Palmer, Melissa S. Roth, et al. Red Fluorescent Protein Responsible for Pigmentation in Trematode-Infected Porites compressa Tissues. Biol. Bull, 2009; 216: 68 - 74.
- Red fluorescent protein variants with incorporated non-natural amino acid analogues, Ann Goulding, Suresh Shrestha, et al. Red fluorescent protein variants with incorporated non-natural amino acid analogues. Protein Eng. Des. Sel, 2008; 21: 101 - 106.
