Caspase-1 Substrate YVAD-AFC

SKU
C-2227
  • Formula:C33H36F3N5O10
  • MW:719.7
  • Appearance:Liquid
  • Purity:99% by HPLC
Product Name Qty
Caspase-1 Substrate YVAD-AFC
200 assays
$284.77
Caspase-1 Substrate YVAD-AFC
1000 assays
$608.61
Ready-to-use fluorometric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate YVAD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large volume caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80), and DTT used for caspase assays are also available separately.
More Information
Alternate Name/Synonyms
Ac-YVAD-AFC, Caspase-1 Substrate, Fluorogenic
SKU
C-2227
Chemical Name
Ac-YVAD-AFC, Caspase-1 Substrate, Fluorogenic
Chemical Formula
C33H36F3N5O10
Molecular Weight
719.7
Appearance
Liquid
Purity
99% by HPLC
Stability
N/A
Storage Temp
-20°C
THERAPEUTIC AREA
Not Available
USE
A fluorogenic substrate for caspase-1 and caspase-4
MDL NUMBER
MFCD01318881
CHEMACX
X1219156-1
INCHI
InChI=1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1
SMILES
C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)Nc1ccc2c(cc(=O)oc2c1)C(F)(F)F)NC(=O)[C@H](C(C)C)NC(=O)[C@H](Cc3ccc(cc3)O)NC(=O)C
Peptide sequence
AC-TYR-VAL-ALA-ASP-AFC
RTECS
Not Applicable
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Protect from light and moisture
Promo text for AGS discount
N/A
GHS PICTOGRAMS
Handling Protect from light and moisture
UN #'S Not Applicable
Packing Group Not Applicable
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