Caspase-9 Substrate LEHD-AFC

SKU
C-2254
  • Formula:C33H38F3N7O11
  • MW:765.7
  • Appearance:Liquid
  • Purity:98% by HPLC
Product Name Qty
Caspase-9 Substrate LEHD-AFC
1000 assays
$608.61
Caspase-9 Substrate LEHD-AFC
200 assays
$284.77
Ready-to-use fluorometric substrate for caspase-9/Mch-6 and related caspases that recognize the amino acid sequence LEHD. Caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) and DTT (Cat.# 1201-1) for caspase assays are also available separately.
More Information
Alternate Name/Synonyms
Ac-LEHD-AFC, Caspase-9 Substrate, Fluorogenic
SKU
C-2254
Chemical Name
Ac-LEHD-AFC, Caspase-9 Substrate, Fluorogenic
Chemical Formula
C33H38F3N7O11
Molecular Weight
765.7
Appearance
Liquid
Purity
98% by HPLC
Storage Temp
-20°C. PROTECT FROM LIGHT!
THERAPEUTIC AREA
Not Available
USE
Fluorometric substrate for caspase-9/Mch-6 and related caspases that recognize the amino acid sequence LEHD
MDL NUMBER
MFCD01862610
CHEMACX
X1271190-9
INCHI
InChI=1S/C33H38F3N7O11/c1-15(2)8-22(39-16(3)44)31(52)41-21(6-7-26(45)46)29(50)42-23(9-18-13-37-14-38-18)32(53)43-24(12-27(47)48)30(51)40-17-4-5-19-20(33(34,35)36)11-28(49)54-25(19)10-17/h4-5,10-11,13-15,21-24H,6-9,12H2,1-3H3,(H,37,38)(H,39,44)(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,45,46)(H,47,48)
SMILES
CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)O)C(=O)NC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F)NC(=O)C
Peptide sequence
AC-LEU-GLU-HIS-ASP-AFC
RTECS
Not Applicable
UN #'S
Not Applicable
PACKING GROUP
Not Applicable
HAZARD CLASS
Not Applicable
Handling
Protect from light and moisture
Certificate of Analysis 1
GHS PICTOGRAMS
Handling Protect from light and moisture
UN #'S Not Applicable
Packing Group Not Applicable
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